Ciprofloxacin depressive effect on a rat model and possible underlying mechanisms | ||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||
| Volume 3, Issue 2, June 2023, Pages 77-84 | ||
| Document Type: Original research articles | ||
| DOI: 10.21608/aijpms.2023.174537.1179 | ||
| Authors | ||
| Nourina N. Ghoneim* 1; Shereen S. Elshaer2, 3; Omyma K. Radwan4; Abeer Salama5 | ||
| 1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt. | ||
| 2Department of Biochemistry, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt. | ||
| 3Department of Biochemistry, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt . | ||
| 4Department of Physiology, Egyptian Drug Authority. | ||
| 5Department of Pharmacology, National Reasearch Centre, El-Buhouth St, Dokki, Cairo 12622. Egypt. | ||
| Abstract | ||
| A fluoroquinolone antibiotic called ciprofloxacin (CPX) is used to treat various bacterial diseases such as respiratory, urinary tract, gastrointestinal, and abdominal infections. This study is aimed to investigate the potential processes by which CPX may promote depression when given at three different doses. At rats, CPX was given orally in dosages of 12.5, 25 and 50 mg/kg per day for 14 days. The forced swimming test was used to assess this depression. Also, we studied the effects of CPX treatment on brain contents of serotonin (5-HT), g-amino-butyric acid (GABA) and excitatory amino acid transporter 2 (EAAT 2). Nitric oxide (NO) and malondialdehyde (MDA) measurements in the brain tissues were used to assess the role that oxidative stress played in the CPX-induced alterations. This study showed that depression-like behavior was only observed in the 50 mg/kg-treated group of CPX at the same time as 5-HT, GABA and EAAT2 levels decreased in the brain. Additionally, increased NO and MDA activity were found in the brain homogenates of the groups that received CPX compared to their controls, which showed increased oxidative stress and a compromised antioxidant defense mechanisms. In conclusion, the 50 mg/Kg CPX dose was more effective than the other two doses at inducing depression because it had a longer effect on swimming time, decreased brain 5-HT, GABA and EAAT 2 levels, and elevated oxidative stress markers; those were considered possible underlying mechanisms of depression caused by CPX. | ||
| Keywords | ||
| CPX; Model of depression; 5-HT; GABA; EAAT2 and Oxidative stress | ||
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