Nagaty Mahrous, M., Attia, E., Abdelkader, M., Hamed, A. (2023). Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne. test, 6(3), 150-154. doi: 10.21608/jabps.2023.198473.1185
Mahmoud Nagaty Mahrous; Eman Zekry Attia; Mohamed Salaheldin A. Abdelkader; Ashraf N. E. Hamed. "Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne". test, 6, 3, 2023, 150-154. doi: 10.21608/jabps.2023.198473.1185
Nagaty Mahrous, M., Attia, E., Abdelkader, M., Hamed, A. (2023). 'Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne', test, 6(3), pp. 150-154. doi: 10.21608/jabps.2023.198473.1185
Nagaty Mahrous, M., Attia, E., Abdelkader, M., Hamed, A. Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne. test, 2023; 6(3): 150-154. doi: 10.21608/jabps.2023.198473.1185

Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne

Journal of Advanced Biomedical and Pharmaceutical Sciences
Article 5, Volume 6, Issue 3, July 2023, Pages 150-154    PDF (450.49 K)
Document Type: Original Article
DOI: 10.21608/jabps.2023.198473.1185
Authors
Mahmoud Nagaty Mahrous* 1; Eman Zekry Attia2; Mohamed Salaheldin A. Abdelkader3; Ashraf N. E. Hamed4
1Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt.
2Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt
3Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, Nasr City, Eastern Avenue, P.O. Box 11432, Sohag, Egypt.
4Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia Egypt
Abstract
The current study aimed to investigate the preliminary phytochemical screening, quantify the total phenolic (TPC), flavonoidal (TFC) contents and evaluate the antioxidant activities of the total methanol extract (ME) of the aerial parts and its derived fractions of Lolium perenne L. (L. perenne). The preliminary phytochemical screening of ME displayed the presence of carbohydrates and/or glycosides, steroids and/or triterpenes, phenolic compounds, proteins and/or amino acids, flavonoids, and quinones. Dichloromethane (DFr) and ethyl acetate (EFr) fractions showed the highest TPC (82.42±2.70 and 70.85±2.27 mg gallic acid equivalent (GAE)/g dried sample), respectively. Also, DFr and EFr displayed the highest TFC (115.65±6.43 and 146.00±1.06 mg rutin equivalent (RE)/g dried sample), respectively. All samples showed variable antioxidant properties. While EFr, followed by DFr, demonstrated the least IC50 value of 120.04±0.43 and 166.81±0.53 µg/mL, respectively, indicating their high antioxidant potential.
Keywords
Lolium perenne; Antioxidant activities; Flavonoidal content; phenolic content; Phytochemical screening
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