P32 Gene PCR Evaluation for Capripox’s Diagnostic Potential and Immunogenic Effect | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Volume 15, Issue 1, June 2023, Pages 523-537 PDF (740.79 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2023.303858 | ||
| Authors | ||
| Zain Alhindi; Afnan Shakoori | ||
| Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia | ||
| Abstract | ||
| Capripoxviruses are a group of extremely contagious viruses that cause substantial economic losses in livestock, especially goats and sheep. The purpose of this investigation was to determine the genetic diversity, antigenicity, and docking analysis of the P32 protein and its epitopes in capripoxviruses. From goat poxvirus (GPV) isolates, the gene encoding P32 was isolated and sequenced. The analysis revealed that the P32 protein is highly conserved across distinct isolates. From the P32 protein sequence, B-cell and T-cell epitopes were predicted using bioinformatics tools. Antigenicity of the predicted epitopes suggests their potential as vaccine targets. The interaction between epitopes and receptor proteins was investigated using a docking analysis. These findings shed light on the binding affinity and stability of epitope-receptor complexes. Additionally, a phylogenetic analysis was performed to determine the genetic relationships between capripoxvirus isolates. The results demonstrated a close relationship between the studied samples and Chinese strains, indicating potential genetic similarities and a shared evolutionary history. Overall, this study contributes to our comprehension of the biology of the capripoxvirus and provides valuable information regarding potential vaccine targets and their interactions with receptor proteins. The findings have implications for the development of effective vaccines and diagnostic tools against capripoxvirus infections, which can aid in the control and prevention of the spread of these economically significant diseases among livestock populations. | ||
| Keywords | ||
| Capripox viruses, Epitopes, OIE diseases; P32 protein, Phylogenetic analysis | ||
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