The Effect of Aliskiren on Renal Cortical Ischemia/Reperfusion Injury in Albino Rats: A Histological and Immunohistochemical Study | ||
| Egyptian Journal of Histology | ||
| Article 6, Volume 42, Issue 4, December 2019, Pages 838-848 PDF (4.9 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2019.10841.1102 | ||
| Authors | ||
| Dalia Ibrahim Ismail* 1; Alshaymaa Gamal Aboulkhair2 | ||
| 1Histology Department, Faculty of Medicine, Cairo University, Cairo, Egypt | ||
| 2Histology Department, Faculty of Medicine, Cairo University | ||
| Abstract | ||
| Background: Ischemia/reperfusion injury (IRI) is a foremost reason for acute kidney injury, which is a common clinical complication having high morbidity rate and lacks effective therapy. Renin-angiotensin system (RAS) activation and angiotensin II level elevation are important risk factors in IRI. Aliskiren (ALS) is the first oral direct rennin inhibitor that blocks the first step in RAS and has been approved for treating hypertension. Aim of work: Investigating whether ALS pretreatment protects the renal cortex of adult male albino rats subjected to IRI. Material and Methods: Thirty adult male albino rats were divided into 3 groups: group I (control group), group II (IRI group): were subjected to IRI procedure, group III (ALS group): rats were pretreated with aliskiren (ALS, 100 mg/kg) twice orally 24h and 1h before IRI and left for 24h of reperfusion before scarification. Blood samples were taken to estimate the levels of blood urea nitrogen (BUN), serum creatinine (SCr) and superoxide dismutase enzyme (SOD). Kidneys sections were stained with HandE and PAS stains and immunohistochemical staining against COX-2 and caspase-3. This was followed by morphometric and statistical analysis. Results: IRI group showed deterioration in renal function tests with massive histological alterations in the Malpighian renal corpuscles and the convoluted tubules. There was significant decrease in optical density of PAS reaction, with significant increase in the mean area% of COX-2 and caspase-3 immunoexpression. ALS group showed significant improvement in renal function tests with preserved normal histology of the renal cortex. There was significant increase in the optical density of PAS reaction, with significant decrease in the mean area% of COX-2 and caspase-3 immunoexpression. Conclusion: A protective effect of ALS was detected in IRI-induced renal cortical damage in adult male albino rats. This was evidenced by laboratory results, histological and immunohistochemical evaluation. | ||
| Keywords | ||
| apoptosis; Inflammation; ischemia/reperfusion injury; Oxidative Stress | ||
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