EFFECTS OF AGE ON SPERM SURVIVABILITY OF THE CAMEL SPERMATOZOA SUPPLEMENTED WITH GLYCINE BETAINE DURING STORAGE AT 5oC. | ||
| Journal of the Egyptian Veterinary Medical Association | ||
| Volume 80, Issue 2, December 2020, Pages 151-174 PDF (435.64 K) | ||
| Document Type: Original Research Articles | ||
| Abstract | ||
| Two experiments were carried out. Twelve Fellahi camels were used in the present study and semen samples were collected using an artificial vagina.The camels were divided into three groups (4 each) according to their ages. The age of the first, second and third groups was <6 to 11, <11 to 16 and <16 to 21 years old, respectively. The first experiment aimed to define the effects of age on copulation time, semen characteristics and blood constituents in the male camel. The second experiment aimed to define the effects of glycine betaine (GB) addition to the cooled semen at levels of 100 and 200 mM on semen quality of the camel at 6 to 11 years, during storage at 5oC for up to 3 days. Semen was diluted with lactose-yolk citrate (LYC) extender.The penetrating ability of spermatozoa supplemented with GB into she-camel cervical mucus, during incubation at 37oC was recorded. The obtained results showed that copulation time (min) and semen-ejaculate volume (ml) were significantly (P<0.05) better in Fellahi camels at <6-11 and <11-16 years than <16-21 years. Semen colour was Creamy white, Creamy White and Milky white, whereas semen consistency was Viscous, Viscous and Semi-viscous of the camels at<6-11, <11-16 and<16-21 years, respectively. However,seminal pH value was insignificantly between different ages of the camels. The percentages of sperm motility and sperm-cell concentration (X106/ ml) were significantly (P<0.05) higher,while the percentages of dead spermatozoa, abnormal spermatozoa, acrosome damage and chromatin damage of the camel spermatozoa at <6-11 and <11-16 years were significantly (P<0.05) lower than camels at <16-21 years. In addition, the effects of age on Total protein, Albumin and Globulin concentrations (gm/100 ml) in the blood serum of the male dromedary camels were not significant. While, Total cholesterol, Potassium, Calcium, phosphorus concentrations (mg/100 ml), Zinc concentration (µg/100 ml) and testosteroneconcentration (ng/100 ml) were significantly (P<0.05) increased of the camels at <6 to 11 and<11 to 16 years compared to those with the camels at <16 to 21 years. Furthermore, Sodium concentration (mg/100 ml) and activity of Aspartate-aminotransaminase (AST) and Alanineaminotransaminase (ALT) enzymes were significantly (P<0.05) higher of the camels at <16 to 21 years than camels at <6 to 11 and <11 to 16 years in the blood serum (Experiment 1). Percentages of sperm motility and storagability of spermatozoa were significantly(P<0.05) higher,while the percentage of dead spermatozoa,abnormal spermatozoa, acrosome damage andchromatin damage of the camel spermatozoaat<6-11yearsadded withGBwere significantly (P<0.05) higher with different storage times at 5oC than free-GB medium. The prolongation of storage time at 5oC was significantly (P<0.05) lower of the camel semen quality either with GB or without GB medium. The penetrating ability of spermatozoa into she-camel cervical mucus was significantly (P<0.05) better with GB than free-GB medium during incubation at 37oC for 4 hours. The advancement of incubation time at 37oCwas significantly (P<0.05) lower of the penetration score for several incubation times either with or without GB addition (Experiment 2). In conclusion, copulation time, semen quality, blood constituents and penetration score were better in camels at <6-11years supplemented with G.B. | ||
| Keywords | ||
| Dromedary camels; Semen quality; Blood; Storage time; Glycine betaine | ||
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