Evaluation and Comparison of Four Screening Tests against Milk Culture for Detection of Subclinical Mastitis in Lactating Cattle and Buffalo in Egypt | ||
| Journal of Applied Veterinary Sciences | ||
| Article 8, Volume 8, Issue 3, July 2023, Pages 67-74 PDF (657.29 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2023.211272.1234 | ||
| Authors | ||
| Ibrahim N. Alkhouly1; Abdelmoneim A. Moustafa2; Nahla A. Abou El Roos1; Sahar A. Kandeel* 2 | ||
| 1Animal health Research Institute, Shibin El Koom branch, Agriculture Research center, Egypt | ||
| 2Department of Animal Medicine (Infectious Diseases), College of Veterinary Medicine, Benha University, Kalyobiya, 13637, Egypt. | ||
| Abstract | ||
| Subclinical mastitis (SCM) is an asymptomatic worldwide udder infection that results in substantial losses to the dairy industry. Our main objective was to evaluate and compare the clinical performance of 4 commercially available screening tests for diagnosing SCM. Foremilk samples were collected from 428 quarters of 107 apparently healthy lactating cows and buffaloes from El-Menofia governorate from 2020 to 2022. Quarter somatic cell count (SCC) was estimated using the FOSS-BacSomatic® counter and the California Mastitis Test (CMT), with SCM defined as SCC > 200,000 cells/mL. Milk pH was measured cow-side using an AD11®pH-meter and BOVIVET® indicator paper. Bacterial cultures of foremilk samples were used to diagnose SCM as a reference method based on the isolation of the causative pathogens. The tests’ performance was evaluated by calculating test sensitivity (Se), specificity (Sp), and accuracy at the optimal-cut-point for each test. FOSS-BacSomatic® counter was the best-performing test for diagnosing SCM (Se = 0.967, Sp = 0.943, accuracy = 0.957) at an optimal-cut-point of >200,000 cells/mL. For comparison, CMT is the second best-performing test at an optimal-cut-point of a non-negative score (Se = 0.892, Sp = 0.878, accuracy = 0.887). The test performance of the AD11® pH-meter and BOVIVET® indicator paper was fair, however, the AD11® pH-meter performed better than the BOVIVET® indicator paper with Se = 0.807, Sp = 0.845, and accuracy = 0.822. We concluded that the FOSS-BacSomatic® counter and CMT are considered good tests for diagnosing SCM. On the other hand, milk pH doesn’t provide a clinically useful method for diagnosing SCM. However, based on cost, availability and analysis time, there doesn't seem to be a persuasive reason to select the FOSS-BacSomatic® counter over the traditional CMT to diagnose SCM. | ||
| Keywords | ||
| California mastitis test; Milk pH; Somatic cell count; Subclinical mastitis | ||
| References | ||
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