Evaluation of some tilapia species as biomarkers for pesticides accumulation levels at Lake Edku, Egypt. | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 17, Volume 23, Issue 2, April 2019, Pages 213-226 PDF (683.83 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2019.30551 | ||
| Author | ||
| Asmaa A. K. | ||
| Abstract | ||
| Three tilapia species; Oreochromis niloticus, Oreochromis aureus and Tilapia zillii were evaluated as biomarkers for five different organophosphorus pesticides (Diazenon, Lindan, Parathion, Malathion and Chlorpyrifos) at Lake Edku, Egypt. O. niloticus reflected highest accumulated pesticides comparing with T. zillii, which reflected lowest accumulation levels of Diazenon, Lindan, Parathion, Malathion and Chlorpyrifos respectively. O. niloticus, O. aureus and T. zillii showed varied antioxidant activity patterns corresponding with pesticide accumulation levels. O. niloticus was superior for Superoxide dismutase activity comparing with O.aureus and T. zillii, which reflected lower level respectively. Moreover, T. zillii reflected the lowest Catalase activity comparing with O. aureus that showed the highest activity. O. niloticus showed high glutathione Peroxidase activity comparing with O. aureus and T. zillii. Glutathione reductase reflected distinguishable variation among O. niloticus, O. aureus, and T. zillii. Glutathione - S-transferase enzyme activity was decreased for T. zillii comparing with O.niloticus and O.aureus. As protein fraction, Glutathione S-transferase (GST) was expressed as one single band with different protein content. Glutathione S-transferase (GST) gene sequence of T. zillii reflected the highest genetic similarity (92.81%) with GST reference sequence. The lowest genetic similarity for GST gene sequence remarked (75.29%) O. niloticus. O. aureus showed moderate genetic similarity (87.11%) comparing with the reference gene sequence. It could be concluded that, significant correlation was detected among different organophosphorus pesticides accumulation and activity levels. Protein content and sequencing of many antioxidant enzymes indicated promising potential of employing three Tilapia species O. niloticus, O. aureus and T. zillii as biomarkers. | ||
| Keywords | ||
| O. niloticus; O. aureus; T. zillii; Organophosphorus pesticides; biomarker; Antioxidant activity; Glutathione S-transferase (GST) | ||
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