Effect of Rhizobium spp. Activated by Colchicine on Morphology, Anatomical Features and DNA Content of Soybean Plant (Glycine Max) | ||
| Scientific Journal of Agricultural Sciences | ||
| Volume 5, Issue 2, June 2023, Pages 93-102 PDF (948.66 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/sjas.2023.211993.1307 | ||
| Authors | ||
| Ragab Ibrahim Elkholy* 1; S.F. Desoukey2 | ||
| 1Alazhar university | ||
| 2Agricultural Botany Department, Faculty of Agriculture, Cairo University, Giza, Egypt | ||
| Abstract | ||
| A significant legume crop known for its high levels of protein and edible oil is the soybean. High levels of nitrogen are needed by soybean plants, and this nitrogen is primarily supplied by biological nitrogen fixation. A field test was performed by using soybean (Glycine max) Giza 82 cultivar.Growing soybean with or without Rhizobium spp. and Colchicine compared to non-inoculated plants, led to raise in some morphological features similar to this, infected plants displayed increased enzyme activity. Such as catalase, polyphenol, peroxidase and ascorbate oxidase,as compared to soybean plants that weren't inoculated . Colchicine concentration was the most crucial source of diversity across all traits. Significant responses were detected for various morphological parameters, anatomical features of root and leaf, and DNA content in the genotype used.High polymorphic bands were found in ISSR tested primers, and fingerprinting revealed 126 distinct DNA fragments in all has 105 fragments on average each primer. There were between one and three polymorphic bands per primer, with an average of 0.75. | ||
| Keywords | ||
| Glycine max; Rhizobium spp.Colchicine, ISSR, Molecular markers | ||
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