HPLC Quantitative Analysis of Two Major Flavonoids and Antimicrobial Effectiveness Against Multi-Drug Resistant Bacteria of Different Parts of Khaya senegalensis | ||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||
| Article 3, Volume 4, Issue 1, January 2024, Pages 32-39 PDF (527.82 K) | ||
| Document Type: Original research articles | ||
| DOI: 10.21608/aijpms.2023.196073.1196 | ||
| Authors | ||
| Amal S Yassin* 1; Salwa A Abu El Wafa1; Nagwan G El Menofy2; Amal H Elmerigy1; Mohamed Marzouk3 | ||
| 1Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy (Girls), Al-Azhar University, P.O. Box 11884, Nasr City, Cairo, Egypt | ||
| 2Department of Microbiology and Immunology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11884, Egypt | ||
| 3Department of Tanning Materials and Leather Technology, Chemical Industries Research Institute, National Research Center National Research Centre, 33 El‑Bohouth St. (Former El‑Tahrir St.), Dokki, Cairo 12622, Egypt. | ||
| Abstract | ||
| Flavonoids are an important group of plant secondary metabolites that are widely used as powerful antioxidants in the management of a variety of illnesses, including inflammatory joint diseases, atherosclerosis, diabetes, in addition to free radical-induced degenerative eye disorders, cancer, dementia, and stroke. The main flavonoids of K. senegalensis plant are quercitrin and rutin. This work aims to determine the quercitrin and rutin content in methanolic extracts of K. senegalensis leaves, stems and fruits in order to determine the most productive part of these bioflavonoids by utilizing a precise HPLC quantitative method. HPLC analysis showed that leaf had the highest yield of quercitrin and rutin among the three parts (2.454 and 0.776 g/kg respectively), the stem had 1.812 and 0.286 g/kg, while fruit had a small amount of quercitrin and had no rutin. Examining certain antibiotic-resistant microorganisms' susceptibility to the antibacterial effects of methanolic extracts of leaves, fruits, and stems concerning the MIC values revealed that the mixture of quercitrin and rutin as the major flavonoids of K. senegalensis in the three different parts the highest degree, Following that are the leaf and stem extracts, however the fruit extract shows no antibacterial action. | ||
| Keywords | ||
| Khaya senegalensis; Meliaceae; quercitrin; rutin; HPLC; antibiotic-resistant bacteria | ||
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