Micro Propagation of Egyptian Taro using Tissue Culture Technique | ||
| Benha Journal of Applied Sciences | ||
| Article 5, Volume 8, Issue 5, May 2023, Pages 55-65 PDF (507.58 K) | ||
| Document Type: Original Research Papers | ||
| DOI: 10.21608/bjas.2023.202809.1137 | ||
| Authors | ||
| S.M.S. Hala1; M.M. ELNagar2; M. H. Mohamed2; L.A. Badr3; M.E. Aliwa4 | ||
| 1Analysis Dept., Nuclear Materials Authority, Kattameya, Egypt. | ||
| 2Horticulture Dept., Faculty of Agriculture, Benha University, Egypt. | ||
| 3Horticulture Dept., Faculty of Agriculture, Benha University , Egypt. | ||
| 4Analysis Dept., Nuclear Materials Authority, Kattameya,Egypt. | ||
| Abstract | ||
| The present study was carried out during the period from 2016 to 2018 for two parts of the experimental work. The first part was in vitro culture and the second part was in vivo culture using the Egyptian local taro cultivar (Colocasia esculenta var. esculenta, Schott.). In vitro experiments were conducted at the plant tissue culture laboratory , Horticulture Department, Faculty of Agriculture, Benha University , to improve number and growth of plantlets in vitro propagation and then to evaluate the field performance of in well adapted taro plants obtained by meristems culture as compared with the traditional propagation methods, i.e. by apical meristems ,leaf pieces ,stem segments and cormal pieces Results indicated that leaf pieces induced 90 % of callus, while stem segments formed 80% of callus .The apical meristem and cormal pieces did not induce callus after 2 weeks of culturing on the (Murashige and Skoog, 1962) MS medium supplemented with ( Benzyl Amino Purine ) BAP and (Naphthalene acetic acid ) NAA and only explants of apical meristem produced shoots (98%). As for the production of callus, apical meristem (zero%), leaves (90%), stems (80%) and cormel (zero %). As for the production of shooting, apical meristem (98%) and the other parts (zero%). These results clearly indicate that among the tested growth regulators types at different concentrations, the apical meristem was the most effective ones to produce the highest number of shoots and leaves explant and the cormel pieces was the least. The treatments BAP (0.20mg/L) and NAA (0.1mg / I) and (40g) sugar gave the highest values of roots number (3.16 and 4.01 roots) , respectively .The lowest treatments in this regard were BAP at (0.2 mg/l), NAA at (0.1mg /L) and 30g sugar as well as control treatment which scored (2.74 and 3.02 roots), respectively. The rest treatments gave roots number values between these two extremes. The results illustrated that the best media for acclimatization was peat moss which produced 100% of survival plants. | ||
| Keywords | ||
| in vitro; tissue culture; shoot multiplication; taro; Kinetin; BAP; NAA | ||
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