Morphological and Molecular characterization of Physical and Chemical Mutations on Durado Plum Cultivar | ||
| Horticulture Research Journal | ||
| Volume 1, Issue 2, June 2023, Pages 148-160 PDF (1.32 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/hrj.2023.307659 | ||
| Abstract | ||
| Improving the marketability of plum cultivars; requires the production of large and high fruits. Breeding by amutation on plums can be used to generate genetic diversity that allows the breeder to screen the mutants for superior quality and quantity fruits. The study was conducted over two-years (2021-2022) in a private farm. It aimed to investigate the impact of various doses of gamma irradiation acting as physical mutagens (20, 30, 40, and 50 Gy) of gamma irradiation from a cobalt (60CO) source, as well asthe effects of (ethyl methane sulphonate EMS) as chemical mutagens with different concentrations at (0.05, 0.1 and 0.2%)on inducing mutations in the Durado plum cultivar. The study on survival percentage after grafting, morphology and molecular characteristics, identifies new mutated genotypes at the molecular level and finally evaluate some of the new genotyping. Utilizing the ISSR technique of compare and relationships between control with Mutagenic plants based on a dendrogram. The results of control gave the highest value on survival percentage after grafting. 40 Gy and EMS 0.2% given the best results in morphology characteristics of the tree in compare with the control. Additionally, the study of relationships and correlation coefficient based on a dendrogram revealed that the relationships between 20 Gy and EMS 0.05% and the control are similar. While 40 Gy and EMS 0.2% is very far in relationship with control. | ||
| Keywords | ||
| Prunussalicina; physical mutagen; chemical mutagen; molecular genetic | ||
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