(2018). BACTERIOLOGICAL AND MOLECULAR INVESTIGATIONS ON CAPRINE MASTITIC MILK WITH SPECIAL REFERENCE ON Corynebacterium pseudotuberculosis. test, 78(3), 325-343.
. "BACTERIOLOGICAL AND MOLECULAR INVESTIGATIONS ON CAPRINE MASTITIC MILK WITH SPECIAL REFERENCE ON Corynebacterium pseudotuberculosis". test, 78, 3, 2018, 325-343.
(2018). 'BACTERIOLOGICAL AND MOLECULAR INVESTIGATIONS ON CAPRINE MASTITIC MILK WITH SPECIAL REFERENCE ON Corynebacterium pseudotuberculosis', test, 78(3), pp. 325-343.
BACTERIOLOGICAL AND MOLECULAR INVESTIGATIONS ON CAPRINE MASTITIC MILK WITH SPECIAL REFERENCE ON Corynebacterium pseudotuberculosis. test, 2018; 78(3): 325-343.

BACTERIOLOGICAL AND MOLECULAR INVESTIGATIONS ON CAPRINE MASTITIC MILK WITH SPECIAL REFERENCE ON Corynebacterium pseudotuberculosis

Journal of the Egyptian Veterinary Medical Association
Volume 78, Issue 3, September 2018, Pages 325-343    PDF (6 M)
Document Type: Original Research Articles
Abstract
The aim of the present study aimed to estimate the prevalence of caprine mastitis with emphasis on Corynebacterium pseudotuberculosis mastitis in Egyptian dairy goats in s farms. Three hundred thirty-six half milk samples were collected from one hundred seventy seven dairy goats of various crossbreeds, in mid to late lactation period after clinical examination. Somatic Cell Count (SCC) and California Mastitis Test (CMT) were assessed in 246 normal half milk samples.One-hundred eighty milk samples (90 clinical and 96 subclinical) were subjected to bacteriological examination, the identified isolets
C. pseudotuberculosis were further confirmed by molecular diagnosis of pld and rpoB genes by PCR. Prevalence of clinical mastitis was 30.5 % (54 animals), while 69.5 % (123 animals) were clinically healthy with normal milk secretion. Out of the selected 246 clinically healthy half milk samples, 96 milk samples (39%) showed subclinical mastitis as detected by SCC
(SCC ≥1,000,000 cells/ml) and California mastitis test scored (+++). The most prevalent bacteria detected in this study were Coagulase negative staphylococci (CNS) 87/186 (46.8 %), Staphylococcus aureus 53/186 (33.9%), E. coli 31/186 (16.7 %), Streptococcus spp. 23/186 (12.4 %) and C. Pseudotuberculosis, 24/186 (12.9%). Molecular detection of C. Pseudotuberculosis virulence genes revealed that gene coding for pld was evidenced in 16 samples of 24 bacteriologically diagnosed samples as C. pseudotuberculosis (66.7%), while gene coding for rpoB was detected in 6/24 samples (25%).
Keywords
Bacteriological investigation; molecular investigation; caprine mastitis; Corynebacterium pseudotuberculosis
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