El Sayed, M., Esawy, A., Sobhey, M., Eidaroos, N., Khattab, R., ELfeky, T., Abdelrahman, M. (2023). Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms. test, 28(1), 157-166. doi: 10.21608/scvmj.2023.307787
Mahmoud El Sayed; Abo Elkheir Esawy; Mona Sobhey; Nada Eidaroos; Rania Khattab; Tamer ELfeky; Mahmoud Abdelrahman. "Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms". test, 28, 1, 2023, 157-166. doi: 10.21608/scvmj.2023.307787
El Sayed, M., Esawy, A., Sobhey, M., Eidaroos, N., Khattab, R., ELfeky, T., Abdelrahman, M. (2023). 'Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms', test, 28(1), pp. 157-166. doi: 10.21608/scvmj.2023.307787
El Sayed, M., Esawy, A., Sobhey, M., Eidaroos, N., Khattab, R., ELfeky, T., Abdelrahman, M. Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms. test, 2023; 28(1): 157-166. doi: 10.21608/scvmj.2023.307787

Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms

Suez Canal Veterinary Medical Journal. SCVMJ
Article 12, Volume 28, Issue 1, June 2023, Pages 157-166    PDF (825.01 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2023.307787
Authors
Mahmoud El Sayed1; Abo Elkheir Esawy2; Mona Sobhey3; Nada Eidaroos1; Rania Khattab* 2; Tamer ELfeky2; Mahmoud Abdelrahman2
1Bacteriology, Immunology and Mycology Department, Faculty of Veterinary medicine, Suez Canal University
2Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)
3Animal Reproduction Research institute.
Abstract
Campylobacter spp < /em>. causes severe issues in chickens. In this investigation. Campylobacter species were isolated (182) in Dakahlia Governorate from 440 broilers and 440 ballade breeds.   Campylobacter coli 42 isolates(4%)and Campylobacter jejuni140isolates (16%). For C. jejuni, rates of recovery from various internal organs were 60%, 24%, 10%, and 5%, from the cloacal swab, heart, liver, and gizzard respectively. The morphological studies revealed that the majority of species from Campylobacter are motile, survive at 37-42Co, and are G-ve, slender spirally curled. They require 5%O2, 85%N2, and 10%CO2 to create a colony that looks like a dew drop on mCCDA media. By using API20, the suspected isolates were identical to typical Campylobacter
Keywords
Campylobacter jejuni; C. coli; Poultry; API 20E and PCR
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