Fabrication and anticancer evaluation of colchicine bound liposomes in Colon Cancer Cell Line | ||
| Egyptian Journal of Chemistry | ||
| Volume 66, Issue 11, November 2023, Pages 565-571 PDF (659.94 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2023.208921.7934 | ||
| Authors | ||
| Nada Hesham Shaarawy* 1; Medhat Wahba Shafaa2; Mahmoud Hammam3; Ahmed Hassan4; Ayman Ibrahim5 | ||
| 1Faculty of science, Helwan university, biophysics department, Helwan, Egypt | ||
| 2Physics Dept., Medical Biophysics Division Faculty of Science Helwan University | ||
| 3Physics, Faculty of Science, Helwan University, Cairo, Egypt | ||
| 4Faculty of science, Helwan university, Botany and microbiology department, Cairo, Egypt. | ||
| 5Faculty of science, Helwan universiy biophysics department Cairo, Egypt | ||
| Abstract | ||
| Treatment of cancer using nanotechnology is an interdisciplinary area of research in science. We intend to use a tumor targeting technique for colchicine-loaded liposomes. Thereafter the attachment of the drug and liposome was studied by transmission electron microscope (TEM) and the drug was successfully encapsulated into the nanoparticles. The size was (318.1 ± 4.44) nm which determined by dynamic light scattering (DLS). In vitro drug release in phosphate buffer saline (0.1M, pH 7.4) showed sustained drug release. Entrapment efficiency of colchicine-loaded liposomes was 97 ± 0.028%. Colchicine-loaded liposomes had an IC50 value of 16.05 g/ml in cytotoxic study utilizing Caco-2 (colon cancer cell line) treated cells, whereas free colchicine had an IC50 value of 25.30 g/ml. When liposomes were utilized in place of free colchicine, the anticancer activity of the drug was increased. | ||
| Keywords | ||
| Liposomes; Colchicine; Nanoparticles; Colon Cancer; Drug delivery system | ||
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