COMPARISON BETWEEN TWO DIAGNOSTIC METHODS FOR DETECTION OF CRYPTOSPORIDIUM SPP. INFECTING FARM ANIMALS IN KUWAIT | ||
| Bulletin of Faculty of Science, Zagazig University | ||
| Article 15, Volume 2016, Issue 1, 2020, Pages 187-193 PDF (184.24 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfszu.2016.31075 | ||
| Abstract | ||
| Cryptosporidiosis is an important disease in young farm animals causing diarrhea and consequently leading to economic losses. In addition, the disease is zoonotic transmitted to humans. Accurate and fast diagnosis is needed for improvement hygienic measures as there is no treatment for cryptosporidiosis. Recently, commercial immunochromatographic (IC) assays have appeared in spite of there are some advantages over the conventional methods e.g. like floatation concentration and Ziehl-Neelsen staining (ZN). This study was conducted to compare between immunochromatographic (IC) assay and ZN, which is widely used routinely in laboratories for diagnosis of Cryptosporidium infection. The Study revealed that IC was a more sensitive detection method than ZN staining. In a comparison of all 1209 fecal samples collected, Cryptosporidium was detected in 12% by IC versus 6.38% by ZN staining (Fishur exact test, P<0.000). Even in each animal species, the number of positive samples detected by IC was higher than those detected by Zn. IC is found to be easy to be performed and its results were easy to be interpret. The overall prevalence of Cryptosporidium infection (16.3%) higher than that of other enteropathogens: rotavirus (2%), coronavirus (0.7%) and E. coli k99 (7.4%). This finding indicated that Cryptosporidium is an important disease agent among farm animals in Kuwait, particularly in cattle and small ruminants. | ||
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