Gold Nanoparticles Alleviate Meloxicam Induced Toxicity in Adult Male Rat Spleen through Activation of Autophagy: Histological and Immunohistochemical Study | ||
| Journal of Medical Histology | ||
| Article 7, Volume 6, Issue 1, June 2022, Pages 92-115 PDF (13.47 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2023.179541.1110 | ||
| Authors | ||
| Sherine Mohammed* 1; Hekmat Abdel Aziz1; Aziz Awaad2; Samira Mohamed2 | ||
| 1Department of Histology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||
| 2Department of Zoology, Faculty of Medicine, Sohag University, Sohag, Egypt | ||
| Abstract | ||
| Background: Meloxicam is an analgesic with higher selective inhibition of cyclooxygenase 2 (COX-2). COX-2 inhibition induces oxidative stress. Gold nanoparticles (AuNPs) have several medical applications in diagnosing and treating diseases and have potent free radical scavenging properties. Objective: This study was conducted to assess the possible therapeutic effect of AuNPs on meloxicam-induced splenic toxicity at different time points. Materials and Methods: Forty-five rats were used in this experiment and were divided into eight groups: group I; the control group. Group II received AuNPs for 2 weeks. Groups III and IV received meloxicam for 2 weeks and 2 months respectively. Groups V and VI received AuNPs for 2 weeks after receiving meloxicam for 2 weeks and 2 months respectively. Groups VII and VIII received meloxicam for two weeks and 2 months respectively followed by cessation of treatment for 2 weeks. Both meloxicam and AuNPs were injected daily intraperitoneally in a dose of 15mg/kg and 50 ul respectively. Histological changes, AuNPs localization in the spleen by silver nitrate, PCNA immunoexpression to detect cellular proliferation, and LC3 and p62 for detecting autophagy activation were studied. Results: Time-dependent degenerative histological changes and increased PCNA, LC3, and p62 expression were observed after meloxicam treatment. However, AuNPs ameliorated these changes. Conclusions: AuNPs have a therapeutic role against the toxic effects of meloxicam in the spleen which may be due to their antioxidant activity, in addition to activation of autophagy. | ||
| Keywords | ||
| AuNPs; Autophagy; Gold nanoparticles; Meloxicam | ||
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