protein engineering design of thermostable insulin for use in various medication | ||
| International Journal of Health Sciences (Egypt) | ||
| Article 2, Volume 2, Issue 3, July 2024, Pages 126-143 PDF (1006.24 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ijhegy.2023.203604.1021 | ||
| Author | ||
| Mohammed M Kassab* | ||
| Instructor of microbiology,immunology,faculty of pharmacy,Cairo university,Egypt Specialist of pharmacology, toxicology and clinical pharmacy. | ||
| Abstract | ||
| Background: Insulin is used to treat type 1 diabetes mellitus, a chronic and severe condition. Many drawbacks exist with subcutaneous insulin injection, such as the fact that it is a thermolability medicine that cannot be kept at room temperature and must instead be kept in a refrigerator (2-8 0C). The aim of the study: Combining bio-informatics and peptidomimetics to create thermostable insulin for use in various medication delivery systems. Type of the study: Experimental investigation of testing. Methodology: In the present study, thermostable insulin was developed that could be kept at ambient temperature away from the refrigerator. In order to manage diabetes mellitus types 1 and 2, insulin is a crucial bio-molecule. Insulin subcutaneous injections have many drawbacks, but the new thermostable insulin has helped to overcome them. Via the inclusion of cysteine and cysteine next to one another in the alpha helices of the core of the two sub-units of insulin, insulin was created utilizing recombinant DNA technology and bio-informatics technology in this study. Trans-dermal drug delivery techniques, such as insulin patches and all forms of insulin injections, as well as other drug delivery systems might be used to create modified thermostable insulin (subcutaneous and intravenous injections). Results: The effectiveness of the test insulin was evaluated using animal models and compared to that of regular subcutaneous insulin. Although the test insulin was thermostable and could be kept at ambient temperature outside of the refrigerator, it was only about 90% as effective as the normal one. | ||
| Keywords | ||
| Thermostable; insulin; drug delivery systems; peptidomimetics | ||
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