Molecular identification of M. bovis BCG by Multiplex PCR | ||
| Benha Veterinary Medical Journal | ||
| Article 19, Volume 31, Issue 1, September 2016, Pages 119-123 PDF (337.59 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2016.31236 | ||
| Authors | ||
| Ashraf A. Abd El-Tawab1; Fatma I. El-Hofy1; Essam A. Nasr2; Nammalwar Sriranganathan3; Enas A. Soliman1 | ||
| 1Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt | ||
| 2Department of diagnostic bacteriology. Veterinary serums and vaccines research Institute, Abbasia, Cairo, Egypt | ||
| 3Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, USA | ||
| Abstract | ||
| Mycobacterium bovis (M. bovis) BCG belongs to Mycobacterium tuberculosis complex (MTBC), highly related organisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other members of MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method for differentiation between members of MTBC. It depends on identification of region of difference (RD) which have been lost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M. tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that is conserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacterium tuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present in all tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains | ||
| Keywords | ||
| Region of difference; BCG; PCR | ||
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