MAJOR VEGETATIVE MORPHOLOGICAL CHARACTERS AND DNA FINGERPRINTS OF TWO MEDIUM CHILLING SWEET CHERRY STRAINS | ||
| Egyptian Journal of Agricultural Research | ||
| Article 11, Volume 80, Issue 4, December 2002, Pages 1623-1634 PDF (5.81 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2002.313155 | ||
| Author | ||
| EMAN S. A. SEIF | ||
| Horticultural Research Institute, Agricultural Research Center, Dokki, Giza, Egypt | ||
| Abstract | ||
| No significant differences in most of the studied characters of stem parts and leaves between the two strains of sweet cherries were detected. However, number of shoots developed on major branches was higher in strain (A) than (B), and colour of branches was slightly different in both studied strains. Shoot lenticels were well more elongated in strain (A) than (B). leaf blade base was wider in strain (B), number of serrates in strain (A) was more than (B), while upper surface in strain (B) is evidently darker in colour. Five random primers (10•mer) were used to amplify genomic DNA from combined leaf samples for each strain. Specific bands were observed with some primers. Three primers namely: (OPA — 10, OPC — 11 and OPN - 13) gave sufficient amplificantion products with clover genome. Primer OPA — 10 gave 7 bands in strain (A) and 9 bands in strain (8). while primer OPC —11 gave only 4 bands in strain (A) and 6 bands in strain (B), while primer OPN — 13 gave 4 bands in strain (A) and 5 bands in strain (B). Thus, DNA fingerprints could be used for the recognization of the studied strains. However, strain (B) could not be considered as good pollenizer for strain (A) as the results show certain genetical relationships. | ||
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