CONCENTRATION, PURIFICATION AND IMMUNOGENECITY OF 146S ANTIGENIC PARTICLES OF FMDV | ||
| Journal of the Egyptian Veterinary Medical Association | ||
| Volume 76, Issue 3, August 2016, Pages 403-410 PDF (1.03 M) | ||
| Document Type: Original Research Articles | ||
| Abstract | ||
| The 146S antigenic mass load is the key success of FMD vaccines.In the current study, concentrated, ultra-filtered and quantified FMD 146S antigens were prepared for immunization of cattle via S/C (sub-cutaneous) and mucosal routes. The immunological reactivity of the prepared antigens was estimated by DOT-ELISA.The coupling of both concentration and ultrafiltration methods, showed the highest 146S quantity peak and the strongest antigenantibody reaction in DOT-ELISA. The SNT results of the immunized cattle revealed that the protective neutralizing antibody response was successfully elicited following single S/C immunization.While, in cattle immunized via mucosal route, slight increase in antibody levels was observed. In conclusion, this study gives an insight to the importance of preparing highly purified and quantified 146S FMD antigens required for inducing potent immune response especially in mucosal vaccines. | ||
| Keywords | ||
| FMDV; 146S antigenic mass; concentration; ultrafiltration | ||
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