Farhadi Mahalli, M., Mortazavi, P., Motesaddi Zarandi, S., Eidi, A. (2023). Investigating The effect of Particulate Matter (PM2.5) On The expression Profile and Methylation of EGR Gene Family and P53 Gene in The lung Tissue of Rat Exposed to Air Pollution. test, 54(6), 1177-1186. doi: 10.21608/ejvs.2023.222617.1540
Mahdi Farhadi Mahalli; Pejman Mortazavi; Saeed Motesaddi Zarandi; Akram Eidi. "Investigating The effect of Particulate Matter (PM2.5) On The expression Profile and Methylation of EGR Gene Family and P53 Gene in The lung Tissue of Rat Exposed to Air Pollution". test, 54, 6, 2023, 1177-1186. doi: 10.21608/ejvs.2023.222617.1540
Farhadi Mahalli, M., Mortazavi, P., Motesaddi Zarandi, S., Eidi, A. (2023). 'Investigating The effect of Particulate Matter (PM2.5) On The expression Profile and Methylation of EGR Gene Family and P53 Gene in The lung Tissue of Rat Exposed to Air Pollution', test, 54(6), pp. 1177-1186. doi: 10.21608/ejvs.2023.222617.1540
Farhadi Mahalli, M., Mortazavi, P., Motesaddi Zarandi, S., Eidi, A. Investigating The effect of Particulate Matter (PM2.5) On The expression Profile and Methylation of EGR Gene Family and P53 Gene in The lung Tissue of Rat Exposed to Air Pollution. test, 2023; 54(6): 1177-1186. doi: 10.21608/ejvs.2023.222617.1540

Investigating The effect of Particulate Matter (PM2.5) On The expression Profile and Methylation of EGR Gene Family and P53 Gene in The lung Tissue of Rat Exposed to Air Pollution

Egyptian Journal of Veterinary Sciences
Volume 54, Issue 6, November and December 2023, Pages 1177-1186    PDF (1.43 M)
Document Type: Original Article
DOI: 10.21608/ejvs.2023.222617.1540
Authors
Mahdi Farhadi Mahalli1; Pejman Mortazavi2; Saeed Motesaddi Zarandi* 3; Akram Eidi4
11Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
22Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
33Department of Environmental Health Engineering, School of public Health and Safety, Shahid Beheshti university of medical sciences, Tehran, Iran.
44Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
Particles smaller than 2.5 μm in size and diameter of particulate matter (PM2.5) are known to be associated with several cancers, lung and cardiovascular disease, cognitive, hormonal and reproductive disorders. The present study investigated the effect of PM2.5 exposure on the expression level and methylation of the transcription factors EGR1 and EGR2 and the well-known proto-oncogene P53 in lung tissue of rat model of air pollution exposure.48 adult Wistar albino rats (190±35 g) were classified into three treatment and control groups. The current study results were showed a significantly low expression of EGR-1, EGR-2, and P53 in both exposure groups (exposure 1 and 2) compared with the control group. Also, significant down expression of EGR-1 and EGR-2 in group exposure one was revealed versus group exposure 2. MS-HRM assays detected hypermethylation of exposure one and exposure two groups compared to control in six months (for EGR-1, EGR-2, and P53). Our findings revealed that PM2.5 exposure could provoke downregulation and hyper-methylation on transcription factors and proto-oncogenes that, in turn, may alter the expression level and function of several essential genes downstream of molecular pathways. PM2.5 exposure could potentially accelerate several respiratory diseases and cancers and should be considered as a health concern.
Keywords
PM2.5 exposure; EGR-1; EGR-2; P53
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