Khater, K., Arafa, S., Selem, G. (2023). Mitochondrial DNA Identification and Ultrastructure of Spermatozoa of the Forensically Important Blowfly, Lucilia cuprina (Diptera: Calliphoridae) in Sharkia Governorate, Egypt.. test, 16(3), 27-44. doi: 10.21608/eajbsa.2023.314110
Karima S. Khater; Salwa Z. Arafa; Gamila Sh. Selem. "Mitochondrial DNA Identification and Ultrastructure of Spermatozoa of the Forensically Important Blowfly, Lucilia cuprina (Diptera: Calliphoridae) in Sharkia Governorate, Egypt.". test, 16, 3, 2023, 27-44. doi: 10.21608/eajbsa.2023.314110
Khater, K., Arafa, S., Selem, G. (2023). 'Mitochondrial DNA Identification and Ultrastructure of Spermatozoa of the Forensically Important Blowfly, Lucilia cuprina (Diptera: Calliphoridae) in Sharkia Governorate, Egypt.', test, 16(3), pp. 27-44. doi: 10.21608/eajbsa.2023.314110
Khater, K., Arafa, S., Selem, G. Mitochondrial DNA Identification and Ultrastructure of Spermatozoa of the Forensically Important Blowfly, Lucilia cuprina (Diptera: Calliphoridae) in Sharkia Governorate, Egypt.. test, 2023; 16(3): 27-44. doi: 10.21608/eajbsa.2023.314110

Mitochondrial DNA Identification and Ultrastructure of Spermatozoa of the Forensically Important Blowfly, Lucilia cuprina (Diptera: Calliphoridae) in Sharkia Governorate, Egypt.

Egyptian Academic Journal of Biological Sciences. A, Entomology
Volume 16, Issue 3, August 2023, Pages 27-44    PDF (916.33 K)
Document Type: Original Article
DOI: 10.21608/eajbsa.2023.314110
Authors
Karima S. Khater1; Salwa Z. Arafa1; Gamila Sh. Selem2
1Zoology Department, Faculty of Science, Zagazig University, Zagazig-44519, Egypt.
2Plant Protection Department, Faculty of Agriculture, Zagazig University, Zagazig -44511, Egypt
Abstract
The blowfly, Lucilia cuprina (Diptera: Calliphoridae) is an important medical and forensic insect that is the main cause of ovine cutaneous myiasis. In the field of medical treatment using maggot therapy, this species could help to heal incurable infections. The morphological identification of the adult wing and Scanning Electron Microscope (SEM) of the first instar larvae was carried out and confirmed by DNA identification of the 3rd instar larvae of L. cuprina. The results showed a fraction of the mitochondrial cytochrome oxidase I (COI) spanning approximately 269 bp. The topology of the Neighbor-Joining (NJ), Maximum Likelihood (ML) tree indicated that L. cuprina species were assigned correctly to the subfamily Luciliinae, family Calliphoridae. The male reproductive system comprises two testes, a pair of vasa differentia and a pair of accessory glands, a vesicula seminalis, and an ejaculatory duct. This inquired species has the common conventional spermatozoal pattern of Calliphoridae, a single-layered acrosome at the apex, a consolidated nucleus, totally crystallized mitochondrial derivatives, and an axoneme with a 9+9+2 microtubular configuration. This investigation revealed for the first time detailed morphological and mitochondrial DNA identification, as well as the morphology of the male reproductive system and ultrastructure of spermatozoa in L. cuprina, collected from Sharkia Governorate, Egypt.
Keywords
COI gene; Mitochondrial DNA; Male reproductive system; Spermatozoa; Scanning Electron Microscope
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