Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines | ||
| Damanhour Journal of Veterinary Sciences | ||
| Volume 10, Issue 1, July 2023, Pages 25-30 PDF (286.17 K) | ||
| Document Type: Original Article | ||
| DOI: 10.5455/djvs.2021.80072.1039 | ||
| Authors | ||
| Asmaa Gamal Abdel-Samad* 1; Nabil Mohamed Bkear2; Adel Mohamed Khadr3; Mohamed Hassan Khodier1; Samir Mohamed Edries1 | ||
| 1Agriculture Research Center (ARC); Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt | ||
| 2Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt | ||
| 3Faculty of Veterinary Medicine, Alexandria University, Egypt | ||
| Abstract | ||
| Through this study, we attempted to prepare and evaluate lyophilized attenuated single and trivalent vaccines of Canine Distemper (CD), Canine Adeno-1 (CA-1), and Canine Parvo (Cp type 2a) viruses. All such preparations were found to be free from foreign contaminants, safe in mice and puppies, and potent, providing vaccinated puppies with good levels of specific antibody titers against the three viruses as estimated by serum neutralization test (SNT) and indirect immune sorbent assay (ELISA). Such antibodies were found to be stable up to 12 months post-vaccination, supporting their use to protect puppies against the three virus infections with the preferable use of the trivalent vaccine, which saves effort and avoids stress factors of vaccinated puppies. | ||
| Keywords | ||
| Canine distemper; Canine Adeno-1; Canine Parvo; single vaccines; combined vaccines; Serum neutralization; ELISA | ||
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