Production and Purification of Heparin Degrading Enzyme from Bacillus Cereus upon Biodegradation of Domestic Chicken Slaughter Byproducts | ||
| Scientific Journal of October 6 University | ||
| Article 3, Volume 3, Issue 1, January 2017, Pages 14-27 PDF (649.77 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/sjou.2017.31571 | ||
| Authors | ||
| Ayman Eldourghamy; Adel K. Khairallah; Abdelhameed M. Othman | ||
| Environmental Biotechnology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI). University of Sadat City | ||
| Abstract | ||
| A Bacillus cereus strain was isolated from Egyptian soil area after a rapid screening in Menufia governorate to select heparinase hyper-producing microbes. The strain gave an enzyme production of 159.6 U/ml after 192 hours of incubation on PYH medium. Chicken slaughter byproducts were used as a supplement for production of pharmaceutical heparinase. The environmental waste was used with different concentrations (1%, 5%, 10%, 15% and 20%) to optimize the process of bioremediation. Enzyme production was gave maximum when using a waste percentage of 15%. Optimum production of the extracellular enzyme was obtained at pH 9.5 and 37oC. Produced enzyme was determined both biochemically and electrochemically in the microbial liquid culture to evaluate the efficiency of the polyion detection method in enzyme detection and both methods gave a similar results. Extracellular heparinase enzyme was purified with gel filtration. Specific activity of the purified enzyme reached 152.12 (U/mg). | ||
| Keywords | ||
| Heparinase; Bacillus; bioremediation; slaughter wastes; polyion detection; gel filtration | ||
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