Molecular Identification and Characterization of Different Fusarium oxysporum f.sp. lycopersici Isolates Using RPAD-PCR Marker | ||
| Journal of Sohag Agriscience (JSAS) | ||
| Volume 8, Issue 1, June 2023, Pages 8-19 PDF (1.55 M) | ||
| Document Type: Research and Review Papers | ||
| DOI: 10.21608/jsasj.2023.316166 | ||
| Authors | ||
| Mahmoud R. Asran1; Moustafa H. A. Moharam1; Eman Zouhry* 2 | ||
| 1Plant Pathology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt | ||
| 2Plant Pathology,Faculty of Agriculture,Sohag University,82524,Sohag,Egypt. | ||
| Abstract | ||
| Five random primers of RAPD-PCR were done in eight isolates of Fusarium oxysporum f.sp. lycopersici, each of them consisted of 10 bases. In accordance with the distinction of the taxa based on RAPD_PCR marker analysis, the results revealed that, the studied isolates of were F. oxysporum f.sp. lycopersici clearly distinguished from each other. Genetic similarity coefficients between pair wise varied from (0.040 to 0.429) based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis of all isolates. The dendrogram obtained from the data showed that, hierarchical clustering separated the isolates into three groups according to their similarity coefficients. Therefore, there were some reliable RAPD markers for the pathogenic isolates, which resulted close relationship at the molecular level under genetic control as it is inherited in a polygenic fashion. Thus, RAPD-PCR technique can be used as an important tool for the genetic identification and characterization of F. oxysporum f.sp. lycopersici species. | ||
| Keywords | ||
| Fusarium; RAPD-PCR; UPGMA; dendrogram | ||
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