An Efficient Method for Regeneration of Stevia rebaudiana (Bertoni) in Egypt | ||
| Alexandria Science Exchange Journal | ||
| Article 10, Volume 33, October-December - Serial Number 4, October 2012, Pages 285-291 PDF (489.45 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/asejaiqjsae.2012.3166 | ||
| Authors | ||
| Marwa M. Ghallab; M. S. Saleh | ||
| Genetics and Breeding Department, Sugar Crop Research Institute, Sabahia Research Station | ||
| Abstract | ||
| Stevia rebaudiana Bertoni, a natural sweetener plant with zero calorie content, becomes an inevitable alternative to sugar especially with the over 346 million diabetic population across the world. The present experiments were conducted to standardize an in vitro culture technique of stevia rebaudiana to explore its potential for callus culture. Leaves explants were cultured on MS medium with specific concentration of growth regulators (2,4-D,BA, Kin and NAA). The results showed that, 1. MS medium containing 1mg/l 2,4-dichlorophexoy acetic acid (2,4-D)+0.5mg/l Benzyl adenine(BA) was gave the best result of callusing. 2. Higher regeneration of plants (32.6 plantlets/callus) was obtained by placing callus on MS medium with 5mg/l BA. 3. Highest rate of root formation (56%) and rooting average (6.1) was recorded in MS medium with 1mg/l IBA. | ||
| Keywords | ||
| Key words: stevia rebaudiana; in Vitro; Regeneration; Callus | ||
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