Evaluation of combined vaccines against bovine brucellosis | ||
| Benha Veterinary Medical Journal | ||
| Article 4, Volume 29, Issue 1, September 2015, Pages 26-31 PDF (225.57 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2015.31785 | ||
| Authors | ||
| El-Olemy G. E.1; Lobna M.A. Salem1; Nashwa O. Khalifa1; El-Ayouby S. M.2; Manal F. Mohamed2 | ||
| 1Department of Zoonosis, Faculty of Veterinary Medicine, Benha University | ||
| 2Veterinary Serum and Vaccine Research Institute, Sera and Antigens Department, Abbasia, Cairo | ||
| Abstract | ||
| Ten female local breed cows proved to be brucella free (six of them were pregnant, parturated and lactate during the experiment time) were subdivided into two subgroups, the first one vaccinated with combination of HS and OMPs subunit vaccines combined with conjunctival vaccination with Br. abortus strain 19 vaccine and the second group was vaccinated with combination of HS and OMPs subunit vaccines beside vaccination with Br. abortus strain RB51 (S/C). From first day post vaccination to 60 days, saliva, vaginal discharge, fecal and milk samples were collected and examined for the presence of the vaccinal strains. Also, blood samples were collected from vaccinated animals and the serum tested serologically using RBPT, MAT and ELISA. In addition, cell mediated immune response was evaluated using Brucellin test. The results revealed that no vaccinal strains was detected in the different body secretions and the humoral immune response of vaccinated cows reached its peak at the 4th week post vaccination then decreased gradually and disappeared at the end of the 10th week. Also, cell mediated immune response revealed that cows vaccinated with the combination of HS and OMPs subunit vaccine combined with conjunctival vaccination with Br. abortus strain 19 vaccine showed remarkable increase in the cell mediated immune response in comparison with in cattle vaccinated with the same combination beside subcutaneous vaccination with Br. abortus strain RB51. | ||
| Keywords | ||
| Bovine brucellosis; humoral immune response; Brucellin test | ||
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