The study of monocyte chemoattractant protein-1 gene expression and ascetic fluid calprotectin biomarker levels in spontaneous bacterial peritonitis patients with liver cirrhosis | ||
| Microbes and Infectious Diseases | ||
| Article 35, Volume 4, Issue 4, November 2023, Pages 1383-1395 PDF (897.55 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2023.227066.1580 | ||
| Authors | ||
| Amany Mohammed Adwy1; Ahmed Mostafa Ashmawy2; Mohammed Saad Badary1; Yossra Mahmoud kamel3; mai ahmed mostafa* 4; Amal Ahmed Elkhawaga1 | ||
| 1Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt | ||
| 2Department of Internal Medicine, Gastroenterology and Hepatology Unit, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt | ||
| 3Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt | ||
| 4Department of Microbiology and Immunology, Faculty of pharmacy, Assiut University, 71515, Egypt | ||
| Abstract | ||
| Background and rationale: Spontaneous bacterial peritonitis (SBP) stands as a primary complication and the most common bacterial infection in individuals suffering from decompensated liver cirrhosis. It is linked to substantial morbidity and mortality rates. The study aimed to determine the level of MCP-1 gene expression in the ascitic fluid of liver cirrhotic patients with or without SBP to evaluate its role in SBP diagnosis and to evaluate the role of ascitic fluid (AF) calprotectin as a diagnostic biomarker for SBP in liver cirrhosis (LC) patients. Methods: A cross-sectional hospital-based study was conducted at Internal Medicine, Gastroenterology and Hepatology Unit at AL-Rajhi Hospital in the period between 2020 and 2022. Participants were divided into two groups, a SBP group (n = 37) and a non-SBP group (LC patients with ascites only) (n = 48). AF levels of MCP-1, calprotectin and blood chemistry tests were estimated in all patients. Results: Both studied groups had no significant differences as regards demographic and laboratory data, except lower potassium levels among the SBP group. Monocyte chemoattractant protein-1 (MCP-1) was significantly higher among the SBP group (p= 0.000) compared to the non-SBP group. Also, both groups had significant differences as regards calprotectin (p= 0.01). Only three patients in the SBP group had positive ascitic fluid cultures (Escherichia coli, Klebsiella pneumonia, and Acinetobacter baumannii) while the non-SBP groups were negative for bacterial growth. Only in the control group, did MCP-1 have a positive correlation with serum albumin and total bilirubin. Conclusion: Ascitic fluid MCP-1 and calprotectin are considered promising biomarkers for early diagnosis of SBP. | ||
| Keywords | ||
| Keywords: ascitic fluid; calprotectin; MCP-1 | ||
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