Induction of Mutations and Genetic Variations in In Vitro of Sour Orange Rootstock (Citrus aurantium) | ||
| Scientific Journal of Agricultural Sciences | ||
| Volume 5, Issue 3, September 2023, Pages 73-92 PDF (1.2 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/sjas.2023.231855.1331 | ||
| Authors | ||
| Fathy G. Hamouda* 1; Hagagy N.A.A1; Abd El-Latif F.M* 1; Bakry Kh.A* 1; Sherif S. Saleh* 2 | ||
| 1Horticulture Department, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, Egypt | ||
| 2Medicinal and Aromatic Plants Research Department., Horticulture Research Institute. Agricultural Research Center, Egypt. | ||
| Abstract | ||
| Micronodes were used to propagate Sour orange (Citrus aurantium) through In Vitro technique. Also, two cytokinins i.e. Kinetin, 6-benzyl amino purine at concentrations rate of 0.0, 1.0, 2.0, & 3.0 mg/l were employed. The mutagenesis process used chemical mutagens through culturing of in vitro shootlets on MS medium supplemented with different concentrations of Sodium azide (NaN3) at rate of 0.04, 0.06, & 0.08%; Colchicine at rate of 0.05, 0.10, & 0.15%. Di methyl sulphanate (DMS( at rate of 0.10, 0.30, & 0.50%; and Di ethyl sulphanate (DES) at rate of 0.10, 0.30, & 0.50%. Also, physical mutagens were subjected to different doses of UV rays ( 2, 4, & 6 hours ); microwave treatments (200 wat) for 10, 20, & 30 seconds; and Gamma rays 50, 75, & 100 Gray. the highest concentration of BAP (3.0 mg/L) is more effective in increasing Shoot numbers. However, the lowest concentration of chemical mutagens i.e. DMS (0.10%) induced the highest Survival% and Shoot length. while, using Sodium azide (0.04, 0.06, & 0.08%) had a harmful effect on Survival% and Shoot length. On the contrary, using Colchicine improved most parameters under study. However, the Vitrification parameter was noticed significantly with all Sodium azide concentrations i.e. 0.08, 0.06, & 0.04% as well as DES 0.30 & 0.50% concentrations as compared with the other treatments. Furthermore, a Molecular marker (ISSR) was done, by Using eight primers revealing that the ratio of polymorphism 81.6% under physical effect was less than compared with chemical mutagenesis 88.4%. | ||
| Keywords | ||
| In Vitro; Citrus; Chemical Mutation; Physical mutation; ISSR | ||
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