Incidence of Staphylococcus aureus and its enterotoxins in yoghurt | ||
| Benha Veterinary Medical Journal | ||
| Article 14, Volume 28, Issue 2, June 2015, Pages 107-114 PDF (266.97 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2015.31875 | ||
| Authors | ||
| Eman F. Mohamed1; Ebtesam M. Mazyed2 | ||
| 1Animal Health Research Institute, Shibin El-kom branch, Food Hygiene Dept. | ||
| 2Animal Health Research Institute, Doki, Giza, Bacteriological, serology Dept. | ||
| Abstract | ||
| A total of 50 samples of balady and automatically packaged plain yoghurt (25 of each) were collected from different dairy shops, groceries and supermarkets at Kalubia Governorates. The collected samples were transferred immediately to the laboratory in an ice box for detection of Staph.aureus,its enterotoxins produced and detection of virulent genes responsible for enterotoxins production. The obtained results revealed that, Staph. aureus could be detected in three samples (12%) and two samples (8%) of examined balady and automatically packaged plain yoghurt, respectively. Isolated strains were tested for detection of enterotoxins producing genes by using PCR. The obtained results showed that one strain belonged to each of balady and automatically packaged plain yoghurt proved to have gene type (A) and (D), respectively. Also detection of enterotoxins by using ELISA revealed that one strain of both balady and automatically packaged plain yoghurt was enterotoxigenic, types (A) and (D) production at percentage of (33.33% and 50%), respectively. The public health importance and hygienic significance of the isolated Staph. aureus and their entertoxins as well as the suggested measures for improving the quality and safety of the yoghurt was discussed | ||
| Keywords | ||
| yoghurt; Staph. aureus; Enterotoxins; PCR | ||
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