Evaluation of the antioxidant and cytotoxic potential of some selected seaweeds: An in vitro study | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 30, Volume 27, Issue 5, September and October 2023, Pages 485-507 PDF (942.59 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2023.319131 | ||
| Author | ||
| Saly F. Gheda et al. | ||
| Abstract | ||
| In this study, four seaweed extracts, Ulva fasciata, Ulva compressa (Chlorophyta), Amphiroa rigida (Rhodophyta), and Sargassum lacerifolium (Phaeophyta) were evaluated for their antioxidant activity. The cytotoxic effects of the seaweed extracts on three different cell lines were also assessed. Using the DPPH (2, 2- diphenyl-1-picrylhydrazyl) scavenging assay, the ethanolic extract of S. lacerifolium had the maximum antioxidant activity (60.17±0.4 %) at 50 µg/ml. Similarly, S. lacerifolium ethanolic extract had the highest ABTS (2,2–azinobis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activity (61.4±0.14 %), at 60 µg/ml. While the total antioxidant capacity of S. lacerifolium extract was recorded (40.19±0.27mg ascorbic acid/gram dry weight (mgAA/gdw). MTT assay for cytotoxicity, in vitro was carried out for the highest antioxidant seaweed S. lacerifolium ethanolic extract, which showed weak cytotoxic activity against MCF7 cell line and moderate cytotoxic activity against HepG2 and Hela cell lines. According to gas chromatography/mass spectrometry (GC/MS) data, S. lacerifolium ethanolic extract displayed the presence of several distinct chemicals, including phytol (8.579%), heptadecane (3.080%), and 1,2,3,4-tetrahydro-1,4,6-trimethylnaphthalene (7.71%). The potential efficacy of the seaweed extract may be related to a synergistic interaction between their fatty acid, alkaloid, phytol, hydrocarbon, phenolic, and phthalate contents, according to GC/MS analyses. In conclusion, these seaweeds are a good source of natural products that contain antioxidant and cytotoxic compounds for the pharmaceutical industry. | ||
| Keywords | ||
| Marine macroalgae; Antioxidant; Cytotoxicity; Gas Chromatography/ Mass Spectroscopy | ||
|
Statistics Article View: 268 PDF Download: 519 |
||
