Mohammed, A., Mohamed, M., Goda, A., Mohamed, S. (2018). Antimicrobial Resistance Pattern of Staphylococcus aureus Isolated from Infected Wounds at Sohag University Hospitals. test, 22(3), 179-187. doi: 10.21608/smj.2018.32146
Alaa El Din Hassan Mohammed; Mona Fattouh Mohamed; Asmaa Mohamed Goda; Samah Raafat Mohamed. "Antimicrobial Resistance Pattern of Staphylococcus aureus Isolated from Infected Wounds at Sohag University Hospitals". test, 22, 3, 2018, 179-187. doi: 10.21608/smj.2018.32146
Mohammed, A., Mohamed, M., Goda, A., Mohamed, S. (2018). 'Antimicrobial Resistance Pattern of Staphylococcus aureus Isolated from Infected Wounds at Sohag University Hospitals', test, 22(3), pp. 179-187. doi: 10.21608/smj.2018.32146
Mohammed, A., Mohamed, M., Goda, A., Mohamed, S. Antimicrobial Resistance Pattern of Staphylococcus aureus Isolated from Infected Wounds at Sohag University Hospitals. test, 2018; 22(3): 179-187. doi: 10.21608/smj.2018.32146

Antimicrobial Resistance Pattern of Staphylococcus aureus Isolated from Infected Wounds at Sohag University Hospitals

Sohag Medical Journal
Article 20, Volume 22, Issue 3, October 2018, Pages 179-187    PDF (124.49 K)
Document Type: Original Article
DOI: 10.21608/smj.2018.32146
Authors
Alaa El Din Hassan Mohammed1; Mona Fattouh Mohamed1; Asmaa Mohamed Goda2; Samah Raafat Mohamed3
1Department of General Surgery, Faculty of Medicine, Sohag University
2Department of Medical Microbiology and immunology, Faculty of Medicine, Sohag University
3Department of Medical Microbiology and immunology, Faculty of Medicine, Sohag University.
Abstract
Background:Staphylococcus aureus is an important human pathogen causing varieties of mild to life threatening community and hospital infections. This study was carried out to determine the antibiotic susceptibility profile of Staphylococcus aureus isolated from infected wounds of patients at Sohag University Hospitals.
patients and methods: 100 clinical isolates of S. aureus were obtained from 350 pus samples using standard microbiological techniques. Antimicrobial susceptibility test was performed using the modified Kirby–Bauer disk diffusion method and confirmed by VITEK 2 automated microbiology system. methicillin resistant Staphylococcus aureus (MRSA) detection was also confirmed by detection of mec A gene by PCR.
Results: S.aureus isolates were totally resistant to Penicillin G and highly  resistant to Cefoxitin and Oxacillin. They were moderately resistant to Gentamycin, Tetracycline, Erythromycin, Clindamycin, Doxycycline and Chloramphenicol.They showed low resistance to Ciprofloxacin, Levofloxacin Trimethoprim/ Sulfamethoxazole, Moxifloxacin and Azithromycin. All isolates were sensitive to Qunipristin/Dalfopristin, Linezolid, Vancomycin ,Tigecycline, Nitrofurantoin, Rifampicin and Teicoplanin.Among the 100 isolated Staphylococcus isolates, 89(89%) were identified as MRSA.
Conclusion: The high incidence of S.aureusisolates resistant to the commonly used antibiotics in the hospital calls for urgent need to put in place measures to curtail the spread of MRSA infections in the hospital.
 
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References
  1. Taddesse, Z., Tiruneh, M., & Gizachew, M. (2014). Staphylococcus aureus and its Antimicrobial Susceptibility Pattern in Patients, Nasal carriage of Health Personnel, and objects at Dessie referral hospital, Northern Ethiopia. Global Journal of Medical Research.‏
  2. Azeez-Akande, O. (2010). Global trend of methicillin-resistant Staphlococcus aureus and emerging challenges for control. African Journal of Clinical and Experimental Microbiology, 11(3).‏
  3. Tong, S. Y., Davis, J. S., Eichenberger, E., Holland, T. L., & Fowler, V. G. (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical microbiology reviews, 28(3), 603-661.
  4. Taylor, T. A., & Unakal, C. G. (2017). Staphylococcus Aureus.‏
  5. Akpaka, P. E., Roberts, R., & Monecke, S. (2017). Molecular characterization of antimicrobial resistance genes against Staphylococcus aureus isolates from Trinidad and Tobago. Journal of infection and public health, 10(3), 316-323.‏
  6. Anstead, G. M., Cadena, J., & Javeri, H. (2014). Treatment of infections due to resistant Staphylococcus aureus. In Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols (pp. 259-309). Humana Press, Totowa, NJ.‏
  7. Kotz, P., Fisher, J., McCluskey, P., Hartwell, S. D., & Dharma, H. (2009). Use of a new silver barrier dressing, ALLEVYN? Ag in exuding chronic wounds. International wound journal, 6(3), 186-194.
  8. Zhang, K., McClure, J. A., Elsayed, S., Louie, T., & Conly, J. M. (2005). Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. Journal of clinical microbiology, 43(10), 5026-5033.‏
  9. Santosaningsih, D., Santoso, S., Setijowati, N., Rasyid, H. A., Budayanti, N. S., Suata, K., ... & Prakoeswa, C. R. (2018). Prevalence and characterisation of Staphylococcus aureus causing community‐acquired skin and soft tissue infections on Java and Bali, Indonesia. Tropical Medicine & International Health, 23(1), 34-44.‏
  10. Bessa, L. J., Fazii, P., Di Giulio, M., & Cellini, L. (2015). Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. International wound journal, 12(1), 47-52.‏
  11. Pires, B. M. F. B., Fuly, P. D. S. C., Ferreira-Carvalho, B. T., & Teixeira, L. A. (2018). Monitoring and Molecular Characterization of Staphylococcus aureus Isolated From Chronic Wounds. Advances in skin & wound care.‏
  12. John, N. P., & Murugan, S. (2014). Biofilm formation by methicillin resistant Staphylococcus aureus and their antibiotic susceptibility pattern: An in vitro study. Current research in bacteriology, 7(1), 1-11.‏
  13. Neopane, P., Nepal, H. P., Shrestha, R., Uehara, O., & Abiko, Y. (2018). In vitro biofilm formation by Staphylococcus aureus isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance. International Journal of General Medicine, 11, 25.‏
  14. Olowo-Okere, A., Atata, R. F., Abass, A., Shuaibu, A. S., Yahya, U. H., & Tanko, N. (2017). Incidence and Antibiotic Susceptibility Profile of Staphylococcus aureus Isolates from Wounds of Patients at Specialist Hospital, Sokoto, Nigeria. Journal of Medical Bacteriology, 6(3, 4), 44-50.‏
  15. Belbase, A., Pant, N. D., Nepal, K., Neupane, B., Baidhya, R., Baidya, R., & Lekhak, B. (2017). Antibiotic resistance and biofilm production among the strains of Staphylococcusaureus isolated from pus/wound swab samples in a tertiary care hospital in Nepal. Annals of clinical microbiology and antimicrobials, 16(1), 15.‏‏
  16. Roy, S., Ahmed, M. U., Uddin, B. M. M., Ratan, Z. A., Rajawat, M., Mehta, V., & Zaman, S. B. (2017). Evaluation of antibiotic susceptibility in wound infections: A pilot study from Bangladesh. F1000Research, 6.‏
  17. Onwubiko, N. E., & Sadiq, N. M. (2011). Antibiotic sensitivity pattern of Staphylococcus aureus from clinical isolates in a tertiary health institution in Kano, Northwestern Nigeria. Pan African Medical Journal, 8(1).‏
  18. Trojan, R., Razdan, L., & Singh, N. (2016). Antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital of Punjab, India. International journal ofMicrobiology, 2016.‏
  19. Gade, N. D., & Qazi, M. S. (2013). Fluoroquinolone therapy in Staphylococcus aureus infections: where do we stand?. Journal of Laboratory physicians, 5(2), 109.‏
  20. Imran, M., Faheem, M., Aslam, V., Hakeem, A., & Shah, A. (2011). Wound infections and culture sensitivity pattern in pediatric burn patients. Journal of Postgraduate Medical Institute (Peshawar-Pakistan), 23(4).‏
  21. Bukhari, S. Z., Ahmed, S., & Zia, N. (2011). Antimicrobial susceptibility pattern of Staphylococcus aureus on clinical isolates and efficacy of laboratory tests to diagnose MRSA: a multi-centre study. Journal of Ayub Medical College Abbottabad, 23(1), 139-142
  22. Gitau, W., Masika, M., Musyoki, M., Museve, B., & Mutwiri, T. (2018). Antimicrobial susceptibility pattern of Staphylococcus aureus isolates from clinical specimens at Kenyatta National Hospital. BMC research notes, 11(1), 226.‏
  23. Rahim, F., Ullah, F., Ishfaq, M., Afridi, A. K., ur Rahman, S., & Rahman, H. (2016). Frequency of common bacteria and their antibiotic sensitivity pattern in diabetics presenting with foot ulcer. Journal of Ayub Medical College Abbottabad, 28(3), 528-533.‏
  24. Yousefi, M., Pourmand, M. R., Fallah, F., Hashemi, A., Mashhadi, R., & Nazari-Alam, A. (2016). Characterization of Staphylococcus aureus biofilm formation in urinary tract infection. Iranian journal of public health, 45(4), 485.
 

 

 

 

 

 

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