Dwedar, S., Hamid, M., Keshk, D., Haroun, A., El Baz, A. (2015). PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM. test, 40(2), 409-417. doi: 10.21608/mjab.2015.322270
Shimaa Dwedar; Marwa S. Abdel- Hamid; Doaa Keshk; A.A. Haroun; A.F. El Baz. "PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM". test, 40, 2, 2015, 409-417. doi: 10.21608/mjab.2015.322270
Dwedar, S., Hamid, M., Keshk, D., Haroun, A., El Baz, A. (2015). 'PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM', test, 40(2), pp. 409-417. doi: 10.21608/mjab.2015.322270
Dwedar, S., Hamid, M., Keshk, D., Haroun, A., El Baz, A. PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM. test, 2015; 40(2): 409-417. doi: 10.21608/mjab.2015.322270

PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM

Menoufia Journal of Agricultural Biotechnology
Article 1, Volume 40, Issue 2, April 2015, Pages 409-417    PDF (847.79 K)
Document Type: original papers
DOI: 10.21608/mjab.2015.322270
Authors
Shimaa Dwedar1; Marwa S. Abdel- Hamid2; Doaa Keshk3; A.A. Haroun4; A.F. El Baz1
1(1) Industerial Biotechnology Dept., Genetic Engineering and Biotechnology Institute, Sadat city
2) Microbial Biotechnology Dept.2, Genetic Engineering and Biotechnology Institute, Sadat city university, Egypt
3(3) Genetic Engineering and Biotechnology Institute , Agriculture Research Center, Giza- Egypt
4(4) Division of Chemical Industries Research, National Research Center, Dokki, Egypt.
Abstract
ABSTRACT: Siderophores are ferric ion specific chelating agents produced by bacteria and fungi growing under low iron stress. The role of these compounds is to scavenge iron from the environment and to make the mineral, which is an essential metal, available to the microbial cells. Azotobacter sp.  isolate Azo-4 isolated from Sadat City ,Egypt showed positive result for siderophores production by Chrome Azurol Sulfonate (CAS) assay. The organism was subjected to various biochemical tests and 16S rRNA analysis; results indicated that the isolated fragment sequence (~1.3) Kbp is homologous to Azotobacter chroococcum strain KCA1 16S ribosomal RNA gene. Deferrated medium concentration induced the siderophores production (45 µM). Hydroxymate siderphore was extracted and the antagonistic activity of partially purified siderphore was tested against bacterial pathogens Bacillus stbtlius and Salmonella sp.,in vitro. Result showed that the Azotobacter sp. under study is a good producer of siderophores, which can be beneficial for its antagonistic activity towards different pathogens as biocontrol agent.
Keywords
Azotobacter chroococcum; Hydroxamate Siderophores; 16SrRNA; SEM; biocontrol
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