Abbas, E., Osman, A., Mohsen, F., Sitohy2, M. (2023). ANTIFUNGAL ACTIVITY OF SOME LEGUME GLYCOPROTEINS AGAINST CUCUMBER FRUIT ROT CAUSED BY Pythium sp.. test, 50(5), 651-663. doi: 10.21608/zjar.2023.323801
Entsar . Abbas; A. Osman; F.S. Mohsen; M. Sitohy2 Sitohy2. "ANTIFUNGAL ACTIVITY OF SOME LEGUME GLYCOPROTEINS AGAINST CUCUMBER FRUIT ROT CAUSED BY Pythium sp.". test, 50, 5, 2023, 651-663. doi: 10.21608/zjar.2023.323801
Abbas, E., Osman, A., Mohsen, F., Sitohy2, M. (2023). 'ANTIFUNGAL ACTIVITY OF SOME LEGUME GLYCOPROTEINS AGAINST CUCUMBER FRUIT ROT CAUSED BY Pythium sp.', test, 50(5), pp. 651-663. doi: 10.21608/zjar.2023.323801
Abbas, E., Osman, A., Mohsen, F., Sitohy2, M. ANTIFUNGAL ACTIVITY OF SOME LEGUME GLYCOPROTEINS AGAINST CUCUMBER FRUIT ROT CAUSED BY Pythium sp.. test, 2023; 50(5): 651-663. doi: 10.21608/zjar.2023.323801

ANTIFUNGAL ACTIVITY OF SOME LEGUME GLYCOPROTEINS AGAINST CUCUMBER FRUIT ROT CAUSED BY Pythium sp.

Zagazig Journal of Agricultural Research
Volume 50, Issue 5, September and October 2023, Pages 651-663    PDF (1.23 M)
Document Type: Original Article
DOI: 10.21608/zjar.2023.323801
Authors
Entsar . Abbas1; A. Osman* 2; F.S. Mohsen3; M. Sitohy2 Sitohy24
1Plant Pathol. Dept., Fac. Agric., Zagazig Univ., Egypt
2Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt
3Hort. Dept., Fac. Agric., Zagazig Univ., Egypt
42. Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt
Abstract
The potential antifungal activity of seed storage glycoprotein (7S globulin) isolated from Cowpea and Lupin seeds against the pathogenic fungus Pythium sp. that cause fruit rot in cucumber was tested in vitroandin vivoon cucumber fruitsafter the harvesting compared to control. The 7S globulin obtained from Cowpea had a high content of total amino acid (78.2%) against 77.27% for 7S globulin obtained from Lupin. Both protein fractions have similar electrophoretic patterns despite different band locations on the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The pathogen was identified in the Plant Pathology Department Lab., Faculty of Agriculture, Zagazig University, as Pythium sp. The mycelial growth of Pythium sp. in liquid media was evidently reduced in the presence of 7S globulin from Cowpea and Lupin in a concentration-dependent manner. The application of 500 µg/mL 7S globulin entirely destabilized and deformed this shape in both compounds (Lupin seed 7S and Cowpea seed 7S). Scanning electron microscopy (SEM) images of the mycelium of Pythium. sp. in inhibition zones induced by either Lupin 7S or Cowpea 7S revealed significant morphological changes in the hyphae, including deformation and contraction. The in vivo tests showed that both Cowpea 7S globulin and Lupine 7S globulin effectively reduced the incidence of postharvest Pythium. sp.infection on cucumber fruits. It can be concluded that there are indeed alternatives to replace the synthetic fungicides for the management of this notorious soil as well as seed-borne fungi (Pythium), which causes big agricultural losses.
Keywords
Fruit rot; legume glycoprotein; Pythium sp; cucumber
Statistics
Article View: 122
PDF Download: 137