Saccharomyces cerevisiae - Fermented Garlic Attenuates Gamma-irradiation Induced Brain and Liver Toxicity Via Regulation of the NF-κB and STAT3 Signaling Pathway | ||
| Egyptian Journal of Radiation Sciences and Applications | ||
| Volume 36, Issue 1, 2023, Pages 33-45 PDF (6.17 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejrsa.2023.236135.1161 | ||
| Authors | ||
| Sawsan M. El-Sonbaty1; Hanan A. Fahmy2; Amel F.M. Ismail* 2 | ||
| 1Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT). Egyptian Atomic Energy Authority, Cairo, Egypt | ||
| 2Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT). Egyptian Atomic Energy Authority, Cairo, Egypt | ||
| Abstract | ||
| The present study was designed to evaluate the radio-protective effects of Saccharomyces cerevisiae-fermented garlic extract (FGE) on γ-irradiation-induced acute toxicity in rats' brain and liver. The rats were divided into four groups, Group I (C; control group) and III (R: irradiated rats) rats administered orally with 1 ml of water. Group II (FGE) and IV (FGE+R): rats administered orally with 1 ml of fermented garlic extract (FGE). Then after 21 days of treatment, rats of groups III, and IV were exposed to a single shot of whole-body gamma irradiation (6 Gy). The results showed that γ-irradiation significantly increased the activity of the hepatic enzymes; ALT, AST, ALP, GGT, and the levels of Total-cholesterol and Triglycerides in the serum. In addition, the activity of SOD, and GSH-Px were significantly decreased, with alteration in the trace elements levels were observed in the brain and liver cytosolic fractions. Moreover, the levels of MDA, and the pro-inflammatory markers; TNF-α, IL-1β, IL-6 and Caspase-3 activity were significantly elevated, GSH content was significantly decreased (p<0.001), the gene expression ratios of NF-κB and STAT3 showed significant up-regulation (p<0.001) as well as DNA fragmentation were also observed in brain and liver tissues. FGE pre-treatment has significantly ameliorated the changes in all these studied parameters. These findings could suggest that the bioactivities of FGE showed neuro-hepato-protective effects on acute toxicity of gamma irradiation by improving the antioxidant system, anti-inflammatory and anti-apoptotic activities associated with regulation of the NF-κB and STAT3 signaling pathway in rats’ brain and liver. | ||
| Keywords | ||
| Antioxidant status; Fermentation; Radioprotection; Trace elements; Yeast | ||
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