NUTRITIONAL AND BIOCHEMICAL STUDIES ON SUPPLEMENTATION OF CHICKPEA POWDER ON RATS DIETS SUFFERED FROM PROTEIN MALNUTRITION | ||
| Menoufia Journal of Food and Dairy Sciences | ||
| Article 3, Volume 40, Issue 3, June 2015, Pages 679-695 PDF (1.14 M) | ||
| Document Type: original papers | ||
| DOI: 10.21608/mjfds.2015.324003 | ||
| Authors | ||
| M. M. E. Ali1; Nabila Y. Mahmoud2; Faten M. M. Abo Zahra2 | ||
| 1(1) Nutrition and Food Science , Home Economics, Menoufia University | ||
| 2(2) Nutrition and Food Science, Faculty of Home Economics, Al- Azhar University | ||
| Abstract | ||
| ABSTRACT: The present study was conducted to investigate the effect of chickpea powder supplementation on rats diet suffered from protein malnutrition in levels of serum blood lipid profile, liver enzymes, renal function, serum proteins and minerals iron and zinc. The current study was performed on a total of forty two normal male albino ratsof an average body weight 150±10 g of Sprague Dawley Strain were used. They were obtained from the Laboratory Animal House of Ophthalmic Research Institute, Giza. Animals were acclimatized to laboratory condition before being used, the rats were divided into two main groups as follows:- The first main group ( 6 rats ) was fed on basal diet and kept as a control negative . the second main group ( 36 rats) subgroup (1): saved as control positive group was fed on a ration only. subgroups were fed(chickpea-cake-cake10%-biscuit-and biscuit 10%).The experiment lasted 4 weeks . Meanwhile , feed intake was calculated every day , while rats weight was recorded weekly . At the end of the experiment , the animals were sacrificed and the blood samples were collected, then centrifuged for 10 minutes at 3000 round per minute " r.p.m " to separate the serum. Also , liver , kidneys , spleen , heart and lung were removed from each rat and cleaned by a saline solution , dried by filter paper and weighed. After that , feed intake , body weight gain , feed efficiency ratio and organs to body weight ratio were calculated .After that, kept Liver and heart in formalin solution for histopathological examination. serum samples were take to determinebiochemical analysis the following : serum liver enzymes(ALT, AST, ALP), total protein and albumin, calculated globulin, albumin/globulin (A/G) ratio, total cholesterol, triglycerides, lipoprotein fractions (HDLc, LDLc and VLDLc),. the obtained results concluded that feeding with chickpea –cake supplementation of chickpea and biscuit supplementation of chickpea improved malnutrition of protein and improved level of iron and zink in the blood serum. | ||
| Keywords | ||
| Chickpea; cake10%; Chickpea 10%; Iron; Zink; live enzymes; lipidprofile | ||
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