Antibacterial resistance and resistance gene detriments of E.coli isolated from chicken | ||
| Benha Veterinary Medical Journal | ||
| Article 31, Volume 28, Issue 2, June 2015, Pages 231-240 PDF (528.39 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2015.32509 | ||
| Authors | ||
| Ashraf A. Abd El Tawab1; Ahmed M. Ammar2; Soad A. Nasef3; Reem M. Reda3 | ||
| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University | ||
| 2Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Zagazig University | ||
| 3Reference Laboratories for Veterinary Quality Control on Poultry Production, Dokki, Giza | ||
| Abstract | ||
| A total of 30 isolate of E.coli isolated from diseased chicken belonged to different serotypes. All isolate showed resistance to one or more antibiotics by disc diffusion methods. They were investigated against 6 antibiotics from different groups the result were, gentamicin about 46.6%, erythromycin 63.3%, tetracycline 80%, ciprofloxacin 40%, ampicellin73.3% and florfenicol 53%.3. Detection of resistance genes by PCR test relieved negative result in all isolated to detected aac(6′ )-Ib-cr gene, while 22 isolate out of 30 examined E.coli isolate were carried the blaTEM gene on anther hand floR gene was detected in 29(96%) of E.coli isolate. Also tetA (A) gene was found in 22(73%) of E.coli isolate out of 30 examined isolate. On another hand aadB (gentamicin resistance gene) gene was present in 8(26%). 11(36%) of E.coli isolate were carried the mph (A) erythromycin resistance gene .Correlation between the presence of resistance gene and the resistance to antibiotics were recorded | ||
| Keywords | ||
| E.coli; Resistance gene; Disc diffusion method; PCR | ||
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