Prospective response of Phaseolus vulgaris seeds primed in silver nanoparticles and aqueous phycocyanin extracted from Spirulina platensis | ||
| Egyptian Journal of Phycology | ||
| Volume 24, Issue 1, 2023, Pages 128-160 PDF (795.31 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/egyjs.2023.240692.1025 | ||
| Authors | ||
| Abdel-Fattah S. Soror1; Mai Walid Ahmed* 2; Samia S. Saffan1 | ||
| 1Department of Botany and Microbiology, Faculty of Science, Zagazig University, Egypt | ||
| 2Botany and Microbiology Department, Faculty of Science, Zagazig University | ||
| Abstract | ||
| The metabolic effects of silver nanoparticles AgNPs prepared by phycocyanin extracted from Spirulina platensis on metabolic activities of economic plant Phaseolus vulgaris including seed germination, pigments(chl. a, chl. b and carotenoids) , carbohydrates, total lipids, amino acid contents, proline, protein banding, malondialdehyde as well as the antioxidant enzymes activities, superoxide dismutase [SOD], peroxidase [APX], and non-enzymatic antioxidants such as glutathione reductase (GR), catalase [CAT] have been investigated. The results demonstrated that all studied parameters seed germination, pigments, carbohydrates, and total lipids were stimulated by low concentrations of AgNPs [5-30 ppm]. On the other hand, the high concentrations of AgNPs [40–50 ppm] exhibited an inhibitory effect .The three antioxidant enzymes SOD, CAT, and GR were increased in a dose-dependent manner as a result of the increase in AgNPs concentrations. The total amino acids progressively increased under the same treatment. Electrophoretic polypeptide banding patterns consists of 159 bands with a molecular weight range of 27 to 145 kDa. Ten bands—represented polymorphic loci with the value of 6.21 %, whereas 6 bands—represented monomorphic loci with value of 3.77%. The most polypeptide bands [14 bands] were discovered in phycocyanin treatments at lane 5 with molecular weights ranging from 27 to 129 KDa as well as AgNPs treatments at lanes 9, 11 and 13 with a value reaching 8.80% and molecular weights ranging from 27 to 145 kDa. | ||
| Keywords | ||
| AgNPs; amino acids; antioxidant enzymes; Phycocyanin; Phaseolus vulgaris and protein banding | ||
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