Bacteriological and molecular studies on staphylococcus aureus isolated from raw milk | ||
| Benha Veterinary Medical Journal | ||
| Article 10, Volume 28, Issue 1, March 2015, Pages 88-97 PDF (359.94 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2015.32658 | ||
| Authors | ||
| Ashraf A. AbdEL-Tawab1; Nahla A. Abou El-Roos2; Asmaa A.M. El-Gendy2 | ||
| 1Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. BenhaUniv. | ||
| 2Animal Health Research “Shebin El- Kom branch” | ||
| Abstract | ||
| The present study was performed on a total of 100 raw buffalo Milk samples from different sources at El-Menofiya Governorate that the inspected samples were taken in ice box within an a hour for bacteriological examination. The results revealed that 80 out of 100 samples (80%) were positive for staphylococcus spp. Out of those 80 samples, 42(53%) were coagulase positive Staphylococci spp while 38(47%) were coagulase Negative Staphylococci spp. Out of those 42 coagulase positive Staphylococci spp,31 were (73.8%) Staph. aureus. In vitro Gentamycin, Trimethoprim / sulphamethazole, Ampicillin, Cephadrine were the most proper antibiotic against isolated Staph. aureus. By using PCR Spa virulence gene was detected in two studied strains while, enterotoxin E was detected only in one strain out of 6 studied strains. Meanwhile, hIyA and enterotoxin (Sea, Seb, Sec, Sed) virulence genes were not detected in all studied strains | ||
| Keywords | ||
| Staphylococcus aureus; Raw milk; PCR | ||
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