N., M., Al-Attraqchi, A., Abdul Abass, A. (2023). Detection of Candidemia in a Sample of Iraqi Neonates Admitted to the Neonates Intensive Care Unit (NICU) by Molecular Methods. test, 9(4), 243-260. doi: 10.21608/jbaar.2023.326813
Mihad Shakir N.; Azhar Abdul Fattah Al-Attraqchi; Areej Abdul Abass. "Detection of Candidemia in a Sample of Iraqi Neonates Admitted to the Neonates Intensive Care Unit (NICU) by Molecular Methods". test, 9, 4, 2023, 243-260. doi: 10.21608/jbaar.2023.326813
N., M., Al-Attraqchi, A., Abdul Abass, A. (2023). 'Detection of Candidemia in a Sample of Iraqi Neonates Admitted to the Neonates Intensive Care Unit (NICU) by Molecular Methods', test, 9(4), pp. 243-260. doi: 10.21608/jbaar.2023.326813
N., M., Al-Attraqchi, A., Abdul Abass, A. Detection of Candidemia in a Sample of Iraqi Neonates Admitted to the Neonates Intensive Care Unit (NICU) by Molecular Methods. test, 2023; 9(4): 243-260. doi: 10.21608/jbaar.2023.326813

Detection of Candidemia in a Sample of Iraqi Neonates Admitted to the Neonates Intensive Care Unit (NICU) by Molecular Methods

Journal of Bioscience and Applied Research
Volume 9, Issue 4, December 2023, Pages 243-260    PDF (1.58 M)
Document Type: Original Article
DOI: 10.21608/jbaar.2023.326813
Authors
Mihad Shakir N.* 1; Azhar Abdul Fattah Al-Attraqchi2; Areej Abdul Abass3
1Department of Physiology, Al-Iraqia University/ College of Medicine -Baghdad, Iraq
2Department Microbiology Medical College/Al Nahrain University, Iraq
3MbchB/CABP professor in pediatric medicine/pediatric department/Medical College/Al Nahrain University, Iraq
Abstract
Candidemia is a leading cause of sickness and mortality in neonatal care. Although current diagnostic methods are beneficial, a better knowledge of molecular pathways is necessary for enhancing detection. This study used molecular techniques to assess the incidence of candidemia in infants being treated in Iraqi NICUs. Using a cross-sectional experimental design, blood samples from newborns exposed to different risk factors were analyzed. The use of polymerase chain reaction (PCR) that targeted the ITS1 and ITS2 regions facilitated the identification of fungi. Several Cladosporium species, such as Cladosporium macrocarpum, Cladosporium allicinum, Cladosporium limoniforme, Cladosporioides, and Cladosporium tenuissimum, were found, which was unexpected. A phylogenetic study indicated the widespread distribution of these strains throughout Asia and North America. Cladosporium's unexpected appearance necessitates a broadening of infection control measures and diagnostic perspectives in healthcare facilities. The findings of this research stress the need for constant vigilance and an all-encompassing approach to infection and diagnosis management in NICUs.
Keywords
Neonatal Candidemia; Cladosporium; ITS1/ITS2 regions; Molecular diagnostics; NICU and Phylogenetic analysis
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