AbouLaila, M., Menshawy, S., Elshafey, B., Osman, A., Yokoyama, N., Igarashi, I., Abdelaziz, A. (2023). Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites. test, 54(7), 211-219. doi: 10.21608/ejvs.2023.242050.1638
Mahmoud AbouLaila; Soad Menshawy; Besheer G. Elshafey; Ahmed E. Osman; Naoaki Yokoyama; Ikuo Igarashi; Amer R. Abdelaziz. "Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites". test, 54, 7, 2023, 211-219. doi: 10.21608/ejvs.2023.242050.1638
AbouLaila, M., Menshawy, S., Elshafey, B., Osman, A., Yokoyama, N., Igarashi, I., Abdelaziz, A. (2023). 'Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites', test, 54(7), pp. 211-219. doi: 10.21608/ejvs.2023.242050.1638
AbouLaila, M., Menshawy, S., Elshafey, B., Osman, A., Yokoyama, N., Igarashi, I., Abdelaziz, A. Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites. test, 2023; 54(7): 211-219. doi: 10.21608/ejvs.2023.242050.1638

Evaluation of The In vitro Inhibitory Effects of Etoposide On The Growth of Babesia and Theileria Parasites

Egyptian Journal of Veterinary Sciences
Volume 54, Issue 7, November and December 2023, Pages 211-219    PDF (1.44 M)
Document Type: Original Article
DOI: 10.21608/ejvs.2023.242050.1638
Authors
Mahmoud AbouLaila* 1; Soad Menshawy1; Besheer G. Elshafey2; Ahmed E. Osman3; Naoaki Yokoyama4; Ikuo Igarashi4; Amer R. Abdelaziz5
1Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Elgomhoria Street, Damanhour22511, Elbehera, Egypt
2Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, El-Behera, Egypt
3Department of Biochemistry, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
4National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
5Department of Parasitology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
Abstract
Etoposide exhibits antibacterial, antimalarial, and anticancer properties. Three Babesia parasites namely, B. bovis, B. bigemina, and B. caballi and Theileria equi were used to assess the remedial impacts of etoposide in vitro. Etoposide’s impact on the transcription of the type II DNA topoisomerase, the DNA gyrase subunits A and B genes, has been studied using reverse-transcription PCR. Etoposide exhibited significant growth inhibition, having IC50 estimates of 3.5 for B. bovis, 4 for B. bigemina, 3.7 for B. caballi, and 4 for T. equi nM. It suppressed growth in B. bovis and T. equi, B. bigemina, and B. caballi at concentrations, including 25 µM and 10 µM, respectively. Throughout the viability assay, it inhibited the regrowth of the four piroplasms at 10 µM. RT-PCR revealed etoposide therapy repressed DNA gyrase transcription in B. bovis. Therefore, the DNA gyrase is the target of etoposide in B. bovis. Etoposide showed promise in treating babesiosis and theileriosis in vitro but requires further studies in vivo evaluation for mouse babesiosis.
Keywords
Etoposide; In vitro; RT-PCR; DNA gyrase
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