El-gazzar, N., Mekki, L., Aboseidah, A., Khalaf, E., badr, A. (2024). SCoT Markers Related to Effects of Gamma Irradiation on some Biochemical Parameters of Fenugreek (Trigonella foenum-graecum L.). test, 8(1), -. doi: 10.21608/fsrt.2023.248050.1113
Nora Shaker El-gazzar; Laila El-Husseiny Mekki; Akram Abdelrahman Aboseidah; El-shahat El-mursi Khalaf; Abdelfattah badr badr. "SCoT Markers Related to Effects of Gamma Irradiation on some Biochemical Parameters of Fenugreek (Trigonella foenum-graecum L.)". test, 8, 1, 2024, -. doi: 10.21608/fsrt.2023.248050.1113
El-gazzar, N., Mekki, L., Aboseidah, A., Khalaf, E., badr, A. (2024). 'SCoT Markers Related to Effects of Gamma Irradiation on some Biochemical Parameters of Fenugreek (Trigonella foenum-graecum L.)', test, 8(1), pp. -. doi: 10.21608/fsrt.2023.248050.1113
El-gazzar, N., Mekki, L., Aboseidah, A., Khalaf, E., badr, A. SCoT Markers Related to Effects of Gamma Irradiation on some Biochemical Parameters of Fenugreek (Trigonella foenum-graecum L.). test, 2024; 8(1): -. doi: 10.21608/fsrt.2023.248050.1113

SCoT Markers Related to Effects of Gamma Irradiation on some Biochemical Parameters of Fenugreek (Trigonella foenum-graecum L.)

Frontiers in Scientific Research and Technology
Volume 8, Issue 1, February 2024    PDF (722.65 K)
Document Type: Original Article
DOI: 10.21608/fsrt.2023.248050.1113
Authors
Nora Shaker El-gazzar* 1; Laila El-Husseiny Mekki2; Akram Abdelrahman Aboseidah3; El-shahat El-mursi Khalaf4; Abdelfattah badr badr5
1botany and microbiology, faculty of science, Suez university
2Botany & Microbiology Department, Faculty of Science, Suez Canal University
3Botany and Microbiology Department, Faculty of Science, Suez University, Suez, Egypt.
4Botany & Microbiology Department, Faculty of Science, Suez University
5Botany & Microbiology Department, Faculty of Science, Helwan University
Abstract
Fenugreek (Trigonella foenum-graecum L.) warrants focused attention by researchers to enhance its value and products. The present article investigates how different gamma irradiation doses (25, 50, 75, 100, and 200 Gy) from cobalt 60 influence biochemical parameters of fenugreek and DNA fingerprinting following exposure to irradiation. Irradiated and unirradiated seeds were grown in the field for two successive seasons, and the impact of the applied treatments on photosynthetic pigments (chlorophyll a, b, and carotenoids) and biochemical parameters, particularly total protein, total amino acids, DPPH radical scavenging activity, total flavonoids, and total phenolics, was examined. Results revealed that the 75 Gy dose exhibited a stimulatory influence on chlorophyll content, carotenoids, and biochemicals, whereas the 200 Gy dose exhibited an inhibitory influence on the same traits. Eleven SCoT primers produced 131 markers, including 67 monomorphic, 48 polymorphic, and 16 unique markers with a mean of 46.91% polymorphism. Polymorphic information content (PIC) varied between 0.30 for the SCoT-1 primer and 0.06 for the SCoT-10 primer. In comparison with the control plants, the results exhibited substantial changes in SCoT profiling in the M2 generation due to γ-irradiation treatments and reveal the significant impacts of lowered gamma irradiation dosages, particularly enhancing chlorophyll content and biochemical parameters in fenugreek plants.
Keywords
Gamma irradiation; Fenugreek; Chlorophyll; Biochemical parameters; SCoT markers
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