Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 08 December 2023 PDF (811.5 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2023.227033.1579 | ||
| Authors | ||
| Babayemi Olawale Oladejo* 1; Adewale Oluwasogo Olalemi1; Oloyede Joshua Oyekanmi2; Success Peter Tabiti1 | ||
| 1Department of Microbiology, Federal University of Technology, Akure, Nigeria. | ||
| 2Inqaba Biotech West Africa, Moniya 200136, Ibadan, Nigeria | ||
| Abstract | ||
| Background: Water quality monitoring is useful for promoting sustainable development of water resources. Objective: The aim of this study was to determine adenovirus concentrations in water samples from River Owena, Nigeria. Methods: The physicochemical characteristics of the water samples were determined immediately after collection using standard methods. A quantitative SYBR green-based real-time PCR assay for detecting human adenovirus-F (HAdV-F) was optimized using DNA extracted with a ZymoBIOMICS™ DNA Miniprep kit from filtered water samples. Results: Findings revealed that HAdV-F genes with end relative fluorescence units (RFUs) ranged from 7206.31 to 14248.55 and corresponding quantification cycles (Cq) ranged from 13.32 to 18.38, while the non-template control (NTC) was 31.71 in a 44-cycle reaction. Water temperature ranged from 34 to 36℃ and pH 6.4 to 7.4, with total solids between 945 and 994 mg/l. Conclusion: Adequate treatment of the water from the river must be a priority by residents who rely on the water for domestic activities. | ||
| Keywords | ||
| Adenovirus; Physicochemical; Water quality; SYBR qPCR | ||
|
Statistics Article View: 259 PDF Download: 97 |
||
