Phytochemical screening and antibacterial activity of Portulaca oleracea L. extracts against antibiotic-resistant bacteria | ||
| Alfarama Journal of Basic & Applied Sciences | ||
| Volume 5, Issue 2, April 2024, Pages 163-174 PDF (1012.22 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajbas.2023.200290.1146 | ||
| Authors | ||
| mohamed abdel-raouf1; Aya Awad Osman* 2; Ahmed I. Abdelkader3; magdy Elbana4 | ||
| 1gastroenterology, surgical center, Mansoura university | ||
| 2Microbiology department, Port said Univercity | ||
| 3Environmental Sciences Department, Faculty of Science, Port Said University, 42526 Egypt | ||
| 4department of botany, faculty of science, port said university | ||
| Abstract | ||
| This study aims to evaluate the antimicrobial activity of the Portulaca Oleracea extracts against some pathogenic bacteria isolated from wound and urine samples. Ethanolic and aqueous extractions of P. Oleracea have been tested for antimicrobial activity against the isolates. The antibacterial activities of both extractions have been compared with some commercial antibiotics. The commonest isolated organisms were Klebsiella Spp. In both urine and wound samples, followed by Escherichia Coli in urine samples, Staphylococcus Aureus in wound samples. The lowest frequent percentage of isolates was Pseudomonas spp. and Proteus Spp. in wound samples. Results showed isolates resistance against tested antibiotics. The inhibition effect of the P. Oleracea extraction is mostly greater than that of antibiotics against Klebsiella Spp. Minimum inhibitory concentration (MIC) of ethanolic extract for Staphylococcus Aureus was 0.35 gm/ml, for E. coli was 0.15 gm/ ml, for Proteus Spp., Pseudomonas spp. and Klebsiella Spp. Was 0.25 mg/ml. For aqueous extract MIC was 0.5 gm/ml for Staphylococcus Aureus and Klebsiella Spp., 0.4 gm/ml for Escherichia Coli, Pseudomonas spp. and Proteus Spp. Phytochemical analysis revealed some component differences for both extracts. The authors are of the opinion that Portulaca Oleracea extractions is a promising substance for controlling some pathogenic bacteria. | ||
| Keywords | ||
| Antibacterial activity; GC Mass; Medicinal plants | ||
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