Effect of Probiotics Versus Silymarin on a Model of Non-Alcoholic Fatty Liver in Adult Male Albino Rats. A Histological Study | ||
| Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry | ||
| Volume 15, Issue 2, December 2023, Pages 133-151 PDF (2.01 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsd.2023.330207 | ||
| Authors | ||
| Samir N. Ibrahim; Nada G. Saied; Ghada G. Hamam; Heba M. Fawzy | ||
| Histology Department, Faculty of Medicine, Ain Shams University, Cairo Egypt | ||
| Abstract | ||
| Background: Non-alcoholic fatty liver disease is the most prevalent widespread liver disease that may progress to liver fibrosis and cirrhosis. Aim: To compare the possible hepatoprotective effects of probiotics versus silymarin on a model of non-alcoholic fatty liver disease in adult male albino rats.Materials and methods: Five equal groups were created by randomly dividing fifty adult albino male rats; group I (control). Rats in the remaining groups received a high-fat, high-fructose diet daily for eight weeks, then they were divided into: groups II in which the animals were sacrificed after 8 weeks and group III in which the animals were left without treatment for a further 4 weeks, groups IV and V were given high fat high fructose diet for 8 weeks then received oral probiotics and silymarin daily (respectively) for further four weeks, then they were sacrificed. Following the experiment, liver samples were exposed to hematoxylin and eosin, Masson`s trichrome, and immunohistochemical techniques including proliferating cell nuclear antigen (PCNA) and hepatocyte-paraffin-1. Histomorphometric studies were also done.Results: A high-fat high-fructose diet resulted in liver vacuolations, a significant increase in collagen deposition, and PCNA expression, while Hepatocyte Paraffin-1 expression showed a significant decrease. Significant improvement of all histological aspects was noticed in probiotics and silymarin-treated groups. However, the silymarin-treated group showed a better effect compared to the probiotic-treated group.Conclusion: administration of both probiotics and silymarin improved Non-alcoholic fatty liver disease. However, silymarin was superior to probiotics. | ||
| Keywords | ||
| Non-alcoholic fatty liver; Probiotics; Silymarin; Hepatocyte paraffin 1; PCNA | ||
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