Herawati, D., Pudjiastuti, P., Zaidan, A., Hendradi, E., Sucipto, T. (2024). Monosaccharides Composition of Fucoidan from Brown Seaweed (Sargassum plagiophyllum) and the Corresponding Antidengue Activity. test, 67(7), 23-29. doi: 10.21608/ejchem.2023.242019.8735
Dheasy - Herawati; Pratiwi Pudjiastuti; Andi Hamim Zaidan; Esti - Hendradi; Teguh Hari Sucipto. "Monosaccharides Composition of Fucoidan from Brown Seaweed (Sargassum plagiophyllum) and the Corresponding Antidengue Activity". test, 67, 7, 2024, 23-29. doi: 10.21608/ejchem.2023.242019.8735
Herawati, D., Pudjiastuti, P., Zaidan, A., Hendradi, E., Sucipto, T. (2024). 'Monosaccharides Composition of Fucoidan from Brown Seaweed (Sargassum plagiophyllum) and the Corresponding Antidengue Activity', test, 67(7), pp. 23-29. doi: 10.21608/ejchem.2023.242019.8735
Herawati, D., Pudjiastuti, P., Zaidan, A., Hendradi, E., Sucipto, T. Monosaccharides Composition of Fucoidan from Brown Seaweed (Sargassum plagiophyllum) and the Corresponding Antidengue Activity. test, 2024; 67(7): 23-29. doi: 10.21608/ejchem.2023.242019.8735

Monosaccharides Composition of Fucoidan from Brown Seaweed (Sargassum plagiophyllum) and the Corresponding Antidengue Activity

Egyptian Journal of Chemistry
Volume 67, Issue 7, July 2024, Pages 23-29    PDF (798.53 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.242019.8735
Authors
Dheasy - Herawati1; Pratiwi Pudjiastuti* 1; Andi Hamim Zaidan2; Esti - Hendradi3; Teguh Hari Sucipto4
1Department of Chemistry, Faculty of Science and Technology, Airlangga University
2Department of Physic, Faculty Science and Technology, Airlangga University
3Department of Pharmaceutical, Faculty of Pharmacy, Airlangga University
4Institute of Tropical Disease, Universitas Airlangga
Abstract
Dengue Fever is an infectious disease caused by dengue virus infection and it poses a significant threat in tropical countries, such as Indonesia. Several studies have shown that the existing treatment options for this condition were ineffective, including the Dengvaxia vaccine released in 2015. This has led to the exploration of bioactive substances with the potential to be developed as antiviral medicines. One of the promising candidates is brown seaweed (Sargassum plagiophyllum), which has been reported to have anti-dengue and cytotoxic effects. Therefore, this study aimed to assess the anti-dengue activity of fucoidan from S. plagiophyllum and analyze its monosaccharides composition. Fucoidan extract was purified with ion exchange chromatography and characterized with H-NMR and HPLC. According to HPLC analysis, the extract consisted of various sugars, including fucose (5980 g/g), galactose, xylose, mannose (1740 g/g), glucose (9.34 g/g), glucuronic, and galacturonic acid (10,06%). Furthermore, the anti-dengue activity was assessed using the viral ToxGlo test and the MTT assay. The results showed that fucoidan had an effective concentration (EC50) of 76.49 g/mL and a selectivity index (SI) of 3.02, indicating the presence of anti-dengue properties.
Keywords
Anti-dengue activity; Brown seaweed; Fucoidan; Sargassum plagiophyllum
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