Mostafa, M., Ibrahim, H., Adel, H., Raghib, H. (2024). Insecticidal and Phytochemical Constituents of Centaurea Aegyptiaca L. against Cotton Aphid, Aphis Gossypii Glov.. test, 67(7), 183-188. doi: 10.21608/ejchem.2023.245267.8792
Mohamed E Mostafa; Heba Y. Ibrahim; Hala Adel; Hanaa M. Raghib. "Insecticidal and Phytochemical Constituents of Centaurea Aegyptiaca L. against Cotton Aphid, Aphis Gossypii Glov.". test, 67, 7, 2024, 183-188. doi: 10.21608/ejchem.2023.245267.8792
Mostafa, M., Ibrahim, H., Adel, H., Raghib, H. (2024). 'Insecticidal and Phytochemical Constituents of Centaurea Aegyptiaca L. against Cotton Aphid, Aphis Gossypii Glov.', test, 67(7), pp. 183-188. doi: 10.21608/ejchem.2023.245267.8792
Mostafa, M., Ibrahim, H., Adel, H., Raghib, H. Insecticidal and Phytochemical Constituents of Centaurea Aegyptiaca L. against Cotton Aphid, Aphis Gossypii Glov.. test, 2024; 67(7): 183-188. doi: 10.21608/ejchem.2023.245267.8792

Insecticidal and Phytochemical Constituents of Centaurea Aegyptiaca L. against Cotton Aphid, Aphis Gossypii Glov.

Egyptian Journal of Chemistry
Volume 67, Issue 7, July 2024, Pages 183-188    PDF (191.22 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.245267.8792
Authors
Mohamed E Mostafa* 1; Heba Y. Ibrahim2; Hala Adel3; Hanaa M. Raghib4
1Plant Protection Research Institute, ARC
2Plant Protection, Research Institute, Agriculture Research Center
3Central Laboratory for organic Agriculture, Agricultural Research Center, Cairo
4Assistant prof. Plant Protection, Research Institute, Agriculture Research Center
Abstract
The chromatographic separation of Centaurea aegyptiaca L. methanolic extract successively yielded four main fractions of different polarities. Among the tested fractions, butanol and ethyl acetate fractions were the most toxic against cotton aphid, A. gossypii using spraying method technique under laboratory conditions after 24 and 72 h of treatment. Application of optimal chromatographic separation of C. aegyptiaca butanol and ethyl acetate fractions afforded six secondary metabolites which were isolated and identified belonging to two main classes flavonol glycosides, the new 6-hydroxy-3-methoxykaempferol-8-O-β-D-glucopyranoside (centaegyin) 1 beside the three 6,8-dihydroxykaempferol-3-O-β-D-glucopyranoside 2, astragalin 3 and populnin 4 those reported from the plant for the first time and two elemanolide sesquiterpenes, methyl 8α-(3,4-dihydroxy-2-methylene-butanoyloxy)-6α,15-dihydroxy-elema-1,3,11(13)-trien-12-oate 5 and methyl 8α,6α,15-trihydroxy-elema-1,3,11(13)-trien-12-oate 6. Assessment of the toxic effect of the isolated compounds, from the active fractions, against A. gossypii revealed that flavonoids 1, 2 and 3 were the active principles in the butanol fraction after 24 and 72 h of treatment. The isolated compounds were structurally identified using various spectroscopic analyses (ESI-MS, 1H-NMR, 13C-NMR, H-H COSY, HSQC and HMBC).
Keywords
Centaurea aegyptiaca; flavonol glycosides; elemanolides; Aphis gossypii
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