The role of Azolla pinnata in hepatic protection and immunity stimulation in broiler chickens

Hamouda, Mohamed S.; Mahrous, Huda A.; Hamza, Hanafy A.; El Moghazy, Gihan M.; Abdel Aal, Mohamed H.

doi:10.21608/javs.2023.249784.1293

	The role of Azolla pinnata in hepatic protection and immunity stimulation in broiler chickens
Journal of Applied Veterinary Sciences
Article 11, Volume 9, Issue 1, January 2024, Pages 105-114 PDF (647.49 K)
Document Type: Original Article
DOI: 10.21608/javs.2023.249784.1293
Authors
Mohamed S. Hamouda¹; Huda A. Mahrous²; Hanafy A. Hamza²; Gihan M. El Moghazy^* ¹; Mohamed H. Abdel Aal¹
¹Regional Center for Food and Feed, Agricultural Research Center, Giza, Egypt
²Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt
Abstract
The aim of the present study was to estimate the phenolic compound and flavonoid contents in Azolla pinnata (A. pinnata) fern extract and evaluate its effect on the liver and immune system in broiler chicks. Mass spectrometry was used to identify the predominant active ingredients. The same study estimated the effect of the detected phytochemicals on the expression of some hepatic protection and immune stimulation-related genes. The experiment was performed using 150 one-day-old Indian River chicks, which were divided into five groups, each containing three replicates. The groups under study were as follows: T1 was fed a corn-based diet with no supplements, T2 was fed a corn-based diet supplemented with 5% A. pinnata sundried fern, T3 was fed a corn-based diet supplemented with 10% A. pinnata sundried fern, T4 was fed a corn-based diet supplemented with 15% A. pinnata sundried fern, and T5 was fed a corn-based diet supplemented with 20% A. pinnata sundried fern. At the end of the experiment, three birds from each subgroup were slaughtered, and their livers were collected to estimate the expression of SOD1, CAT, ACC, LPL, IL8, IL10, and TLR2 genes using real-time PCR. The obtained results showed that all treatments had a significant effect on the tested genes, as they caused up-regulation of their expression, indicating that these genes have antioxidant and immunostimulatory effects. More research is needed to correlate the recommended inclusion rates of A. pinnata with other perf
Keywords
Azolla pinnata; phenolic compounds; flavonoid; hepatic protection; PCR and gene expression

References
AL-SHWILLY, H. A. J. 2022. Azolla as a New Dietary Source in Broiler Feed: A Physiological and Production Study. Archives of Razi Institute, 77(6): 2175-2180. ABD EL-GHANY, W. A. 2020. A review on the use of Azolla species in poultry production. J. World Poult. Res. 10(2): 378-384. https://dx.doi.org/10.36380/jwpr.2020.44. ABD ELRASOUL, A. S., MOUSA, A. A., SAHAR, H. ORABI, MOHAMED, M. A., GAD-ALLAH, S. M., ALMEER, R., ABDEL-DAIM, M. M., SHADEN, A. K., EL-SEEDI, H. R., and ABD ELDAIM, M. A., 2020. Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Effects of Azolla pinnata Ethanolic Extract against Lead-Induced Hepatotoxicity in Rats. Antioxidants, 9: 1014. https://doi.org/10.3390/antiox9101014. AHMADIPOUR, B., HASSANPOUR, H., and KHAJALI, F., 2018. Evaluation of hepatic lipogenesis and antioxidant status of broiler chickens fed mountain celery. BMC veterinary research, 14(1):1-7. https://doi.org/10.1186/s12917-018-1561-6. AVIAGEN, 2018. Indian River Broiler Management Handbook. CHAGAS, M. D. S. S., BEHRENS, M. D., MORAGAS-TELLIS, C. J., PENEDO, G. X. M., SILVA, A. R., and GONÇALVES-DE-ALBUQUERQUE, C. F., 2022. Flavonols and Flavones as Potential Anti-Inflammatory, Antioxidant, and Antibacterial Compounds. Oxid Med Cell Longev. 2022 Sep 6; 2022:9966750. https://doi.org/10.1155/2022/9966750. EGEA, G., JIMÉNEZ-ALTAYÓ, F., and CAMPUZANO, V., 2020. Reactive Oxygen Species and Oxidative Stress in the Pathogenesis and Progression of Genetic Diseases of the Connective Tissue. Antioxidants 9, 1013. https://doi.org/10.3390/antiox9101013. ELNAGAR, R., ELKENANY, R., and YOUNIS, G., 2021. Interleukin gene expression in broiler chickens infected by different Escherichia coli serotypes. Vet World. Oct;14(10):2727-2734. https://doi.org/10.14202%2Fvetworld.2021.2727-2734. EUROPEAN UNION DIRECTIVE., 2010. The protection of animals used for scientific purposes: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=celex%3A32010L0063 IBRAHIM, S., ATEYA, A., and ABDO, M., 2023. Economic Evaluation of Using Azolla on Growth Performance of Broiler Chickens: Gene Expression Impact. Egypt. J. Vet. Sci. 55(1): 29-40. https://doi: 10.21608/EJVS.2023.215995.1519 ISLAM, M. A., (2017). Effect of Azolla (Azolla Pinnata) on Growth and Lipid Profiles of Broiler Chickens. Annals of Bangladesh Agriculture 21 (1 & 2): 73–78. JAFRI, S. A. A., KHALID, Z. M., KHAN, M. Z., and JOGEZAI, N., 2022. Evaluation of phytochemical and antioxidant potential of various extracts from traditionally used medicinal plants of Pakistan. Open Chemistry, vol. 20, no. 1, 2022, pp. 1337-1356. https://doi.org/10.1515/chem-2022-0242. KAMBOH, A. A., HANG, S. Q., KHAN, M. A., and ZHU, W. Y., 2016. In vivo immunomodulatory effects of plant flavonoids in lipopolysaccharide-challenged broilers. Animal. 2016 Oct;10(10):1619-25. https://doi.org/10.1017/S1751731116000562. KAMEL, E. R., and HAMED, E., 2021. Effect of dried Azolla on growth performance, hematological, biochemical, antioxidant parameters, and economic efficiency of broiler chickens. Adv. Anim. Vet. Sci., 9(11): 1886-1894. http://dx.doi.org/10.17582/journal.aavs/2021/9.11.1886.1894. KUMARI, A., KRISTENSEN, K. K., PLOUG, M., and WINTHER, A. L., 2021. The Importance of Lipoprotein Lipase Regulation in Atherosclerosis. Biomedicines. Jul 6;9(7):782 https://doi.org/10.3390/biomedicines9070782. LIU, S., LAI, J., FENG, Y., ZHUO, Y., ZHANG, H., CHEN, Y., LI, J., MEI, X., ZENG, Y., SU, J., DENG, Y., JIANG, F., YANG, S., TAN, H., HON, C. T., WEI, S., HAN, Z., WANG, F., and ZHONG, W., 2023: Acetyl-CoA carboxylase 1 depletion suppresses de novo fatty acid synthesis and mitochondrial β-oxidation in castration-resistant prostate cancer cells. J Biol Chem. Jan;299 (1):102720.:https://doi.org/10.1016/j.jbc.2022.102720. LUMSANGKUL, C., LINH N. V., CHAIWAN, F., ABDEL-TAWWAB, M., DAWOOD, M., FAGGIO, C., JATURASITHA, S., and VAN DOAN, H., 2022. Dietary treatment of Nile tilapia (Oreochromis niloticus) with aquatic fern (Azolla caroliniana) improves growth performance, immunological response, and disease resistance against Streptococcus agalactiae cultured in bio-floc system Aquaculture Reports 24, 101114. https://doi.org/10.1016/j.aqrep.2022.101114. MAHFOUZ, S., SHANG, Q., and PIAO, X., 2021. Phenolic compounds as natural feed additives in poultry and swine diets: a review. J Animal Sci Biotechnol 12, 48. https://doi.org/10.1186/s40104-021-00565-3. MISHRA, D. B., ROY, D., KUMAR, V., BHATTACHARYYA, A., KUMAR, M., KUSHWAHA, R., and VASWANI, S., 2016. Effect of feeding different levels of Azolla pinnata on blood biochemicals, hematology and immunocompetence traits of Chabro chicken. Veterinary World, 9(2), 192. https://doi.org/10.14202/vetworld.2015.192-198. NAWAZ, N. A. S., SYED, J., DILEEP, N., RAKESH, K. N., and PRASHITH KEKUDA, T. R., 2014. Antioxidant activity of Azolla pinnata and Azolla rubra–A comparative study. Sch Acad J Biosci, 2(10), 719-23. NAYAK, N., and PADHY, R. N., 2017. GC-MS analysis of bioactive compounds and host-toxicity studies of Azolla caroliniana symbiotic with the cyanobacterium Anabaena azollae. Indian J Pharm Educ, 51(2S), S24-S33. https://doi.org/10.5530/ijper.51.2s.46. PÉREZ-CANO, F. J., MASSOT-CLADERA, M., RODRÍGUEZ-LAGUNAS, M. J. and CASTELL, M., 2014. Flavonoids Affect Host-Microbiota Crosstalk through TLR Modulation. Antioxidants. 3, 649-670. https://doi.org/10.3390/antiox3040649. PFAFFL M.W. 2001. Development and validation of an externally standardised quantitative insulin like growth factor-1 (IGF-1) RT–PCR using LightCycler SYBR® Green I technology. In Meuer,S., Wittwer,C. and Nakagawara,K. (eds), Rapid Cycle Real-time PCR, Methods and Applications. Springer Press, Heidelberg, Germany pp. 281–291. http://dx.doi.org/10.1007/978-3-642-59524-0_30. PIRAHANCHI, Y., ANORUO, M. D., and SHARMA S., 2023. Biochemistry, Lipoprotein Lipase. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK537040/ PRABINA BJ., and KUMAR K., 2010. Dried Azolla as a nutritionally rich cost effective and immune - modulatory feed supplement for broilers. Asian J. Anim. Sci. 5: 20-22. RASHAD, S., 2021. An overview on the aquatic fern Azolla spp. as a sustainable source of nutrients and bioactive compounds with resourceful applications. Egyptian Journal of Aquatic Biology and Fisheries, 25(1): 775-782. http://dx.doi.org/10.21608/ejabf.2021.150205. REFAEY, M. M., MEHRIM, A. I., ZENHOM, O. A., AREDA, H. A., RAGAZA, J. A., and HASSAAN, M. S., 2023. Fresh Azolla, Azolla pinnata as a Complementary Feed for Oreochromis niloticus: Growth, Digestive Enzymes, Intestinal Morphology, Physiological Responses, and Flesh Quality. Aquaculture Nutrition, 2023. https://doi.org/10.1155/2023/1403704. REHMAN, M. S., REHMAN, S. U., YOUSAF, W., HASSAN, F. U., AHMAD, W., LIU, Q., and PAN, H., 2021. The Potential of Toll-Like Receptors to Modulate Avian Immune System: Exploring the Effects of Genetic Variants and Phytonutrients. Front Genet. Aug 26; 12:671235. https://doi.org/10.3389/fgene.2021.671235 SHUKLA, M., BHATTACHARYYA, A., SHUKLA, P., ROY, D., YADAV, B., and SIROHI, R., 2018. Effect of Azolla feeding on the growth, feed conversion ratio, blood biochemical attributes and immune competence traits of growing turkeys. Veterinary World, 11 (8): 2231-0916. https://doi.org/10.14202/vetworld.2018.459-463. SOŃTA, M., REKIEL, A., and BATORSKA, M., 2019. Use of duckweed (Lemna L.) in sustainable livestock production and aquaculture–a review. Annals of Animal Science, 19(2), 257-271. https://doi.org/10.2478/aoas-2018-0048. SOCIETY FOR ANIMAL RIGHTS IN SCIENCE (SAIRS)., 2023. The Animals in Science Regulation Unit., the Animals (Scientific Procedures) Act 1986 in England, Scotland and Wales. https://www.gov.uk/government/collections/animals-in-science-regulation-unit SREENATH, K. B., SUNDARAM, S., GOPALAKRISHNAN, V. K., and POORNIMA, K., 2016. Quantitative phytochemical analysis, in vitro antioxidant potential and gas chromatography-mass spectrometry studies in ethanolic extract of Azolla microphylla. Asian J. Pharm Clin. Res., 9(2): 318-323. SWAIN, B. K., NAIK, P. K., and BEURA, C. K., 2022. Nutritive value of azolla as poultry feed-a review. Indian Journal of Animal Nutrition, 39(1): 1-11. http://dx.doi.org/10.5958/2231-6744.2022.00001.9. THIRIPURASUNDARI, B., and PADMINI, E., 2018. Preliminary phytochemical screening and evaluation of antimicrobial and antioxidant activity of Azolla pinnata. Int J Recent Sci Res, 9(5F): 26924-26930. http://dx.doi.org/10.24327/ijrsr.2018.0905.2151. WANG, Y., BRANICKY, R., NOË, A., and HEKIMI, S., 2018. Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. J Cell Biol. 2018 Jun 4;217(6):1915-1928. https://doi.org/10.1083/jcb.201708007.
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