Antifungal resistance profiles of Candida isolates from pediatric tertiary care hospital and in vitro efficacy of natural oils against fluconazole-resistant species | ||
| Microbes and Infectious Diseases | ||
| Article 37, Volume 5, Issue 1, February 2024, Pages 359-369 PDF (702.17 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2023.252518.1693 | ||
| Authors | ||
| Heba Hassan Ahmed1; Yousseria M shetaia1; Ashraf M Morgan2; Marwa A Ibrahim* 3; Noha Salah Soliman4 | ||
| 1Microbiology (Mycology) Department, Faculty of Science, Ain-Shams University, Egypt. | ||
| 2Toxicology and Forensic Medicine Department, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||
| 3Biochemistry and Molecular biology Department, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||
| 4Clinical and Chemical Pathology (Microbiology) Department, Faculty of Medicine, Cairo University, Egypt. | ||
| Abstract | ||
| Background: Candidiasis is considered the most significant fungal infection with increasingly reported antifungal resistance, especially against fluconazole. Aim: This study aimed to determine rates of antifungal resistance among Candida isolates from pediatric clinical samples, and test the in-vitro efficacy of natural essential oils against fluconazole-resistant isolates. Methods: Identification and antifungal susceptibility testing were performed utilizing the Vitek2 compact automated system. Fluconazole-resistant Candida isolates were subjected to in-vitro screening for the efficacy of nine natural oils by disc diffusion method, followed by determining the minimal inhibitory concentration for the most effective oils using broth micro dilution. Results: Out of total cultured samples (n=2120), Candida were isolated at a rate of 6.27% (n= 133), mostly from urine and blood samples. Candida albicans was the most prevalent isolated species (54.9%), followed by Candida tropicalis (33.83%). Fluconazole recorded a higher resistance rate (9%) than the rest of antifungals, with a significantly higher incidence among non-C. albicans Candida species (NCAC) than C. albicans (p <0.05). Cinnamon, cumin, thyme, and lemongrass showed the widest mean of inhibition zones, of which cinnamon oil had the lowest MIC values against Candida isolates. Conclusions: Our study concluded higher predominance of C. albicans over NCAC species, and total fluconazole resistance rate of 9%. cinnamon oil was found to be the most effective oil against fluconazole-resistant isolates. | ||
| Keywords | ||
| Candida; Antifungal; Fluconazole resistance; plant oils | ||
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