FRACTIONATION AND CHARACTERIZATION OF PROTEINS OF SOME EGYPTIAN FIELD CROPS | ||
| Zagazig Journal of Agricultural Research | ||
| Article 19, Volume 46, Issue 2, March and April 2019, Pages 475-487 PDF (1.7 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjar.2019.33402 | ||
| Authors | ||
| Zakaria A. Nofel* 1; M. G. Taha1; H. E. Ali1; N. N. Darwish2 | ||
| 1Biochem. Dept., Fac. Agric., Al Azhar Univ., Cairo, Egypt | ||
| 2Chem. at Cairo Univ. Res. Park (CURP), Egypt | ||
| Abstract | ||
| The proteins considered a true expression of genetic characteristics in plant crops and can be used for classifying of many varieties of different crops by using protein electrophoreses SDS PAGE, to fractionate and characterize proteins. Results showed that barley crop whose varieties had a protein C-hordein with molecular weight 21KDa which characterized in each variety but differ in its percentage. Corn crop varieties were characterized by presence of zien protein with molecular weight 19, 17 and 11KDa. While in rice crop, varieties were differed according to protein bands especially in tublin protein with molecular weight 48KDa. Also, the varieties of wheat crop were differed by percentage of some essential protein gliadin: gamma-beta-alpha, with molecular weight 58,42 and 35KDa, respectively. On the other hand, legumes crops: chickpea, faba bean and lentil proteins could be characterized with a lot of protein bands and had been noticed greet difference in kinds of protein or in its percentage of different varieties through molecular weight from 245KDa (Gamma globulin protein) to 11KDa Vicilins proteins. While in fiber crops proteins: cotton and flax were had a less protein bands which were appeared in some varieties of flax protein whose had one protein band (conlinin) with molecular weight 18KD, also cotton protein varieties had different percentages of 7S vicilins proteins with molecular weight 49 and 46KDa. Although oil crops: peanuts, soybean and sunflower proteins had clear differences in all varieties as number of protein bands and/or percentages. Peanuts varieties had the same protein bands their weights from 88KDa (glutenin) to 19KDa (Conarachin) but as a different percentages in each variety. In soybean proteins varieties there were differences in number of protein bands and percentages, sunflower varieties had almost the same protein bands but differed in their percentages and their weights were between 245KDa to 11KDa. Results confirmed that protein electrophoreses could be used as a simple method of classification of filed crop varieties. | ||
| Keywords | ||
| Field corps; electrophoreses; SDS PAGE; cereals; Legumes; fibers; oils; proteins | ||
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